Photosynthetic Trait-Based Evaluation of Lentil Genotypes Under Salt Stress
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Authors
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Pooja Kanwar Shekhawat
Sardarkrushinagar Dantiwada Agricultural University, Banaskantha.Sardarkrushinagar - 385506. Gujarat,India
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Vivek Singh
ICAR-Central Soil Salinity Research Institute, Karnal 132001, India
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Ankit Kumar
ICAR-Central Soil Salinity Research Institute, Karnal 132001, India
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Zeetendra Singh
ICAR-Central Soil Salinity Research Institute, Karnal 132001, India
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Jogendra Singh
ICAR-Central Soil Salinity Research Institute, Karnal 132001, India
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Vijayata Singh
ICAR-Central Soil Salinity Research Institute, Karnal 132001, India
Keywords:
Lentil, Diversity, Salt Tolerance, Photosynthesis, TranspirationAbstract
Lentil (Lens culinaris, Medik.), a nutritionally rich pulse crop, is susceptible to soil salinity. At an electrical conductivity (EC) of up to 5 dS m-1 (~50 mM NaCl), the crop experiences a significant reduction in yield of approximately 90%. The current emphasis on identifying lentil germplasm with salinity tolerance is essential for ensuring super-food production. Assessment of diversity panels for their ability to withstand salt stress is a leading approach for developing breeding lines and salt-tolerant varieties. One hundred diverse lentil genotypes were evaluated for photosynthetic traits under non-saline (control) and saline (EC = 5 dS m-1) conditions. This study was conducted at ICAR-Central Soil Salinity Research Institute, Karnal, during rabi seasons of 2021-22 and 2022-23. Across genotypes. a significant reduction was noticed under salinity in comparison to control for various parameters viz. photosynthetic rate (36.4%), transpiration rate (34.0%), stomatal conductance (34.0%), instantaneous water use efficiency-iWUE (3.7%), chlorophyll content (27.8%), (relative water content) (30.0%), and MSI (membrane stability index (19.0%). The stepwise regression approach revealed that E (Leaf Transpiration Rate), gsw (Stomatal Conductance to Water Vapor), iWUE, inWUE (intrinsic water use efficiency), Chlorophyll, RWC and MSI contributed critically in combating salt stress. A noteworthy positive correlation was noted between Pn, iWUE as well as inWUE under both control and salinity whereas gsw, Pn (Net Photosynthetic Rate), E, and MSI under salinity. Out of eight PCs, initial three principal components (PCs) showed substantial variation. These principal components accounted for 64.6% and 72.2% of the overall variation under normal and saline conditions, respectively. This study provides the fundamental knowledge about how photosynthetic traits respond to salt stress in lentils. The information obtained here will aid in understanding photosynthetic alterations under salt stress.
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