REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR) BASED DETECTION OF PVSO AND PVSA STRAINS BY USING DEGENERATE PRIMERS


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Authors

  • Nimisha Kaushal
  • S J Gawande
  • Priyanka Kaundal
  • I D Garg

Abstract

Reverse transcriptase polymerase chain reaction (RT-PCR) procedure for the detection of both the strain groups PVSo and PVSA of PVS was developed and validated by testing isolates of both the strains as well as mericlones of potato variety Kufri Chipsona-3. Sensitivity and reliability of the technique was compared with that of ELISA and immuno electron microscopy (IEM). The technique was more sensitive and reliable than ELISA, but at par with IEM.

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Author Biography

  • I D Garg
    Division of Plant Protection,Central Potato Research Institute, Shimla 171001, HP, India

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Issue

Vol. 34 No. 1 - 2 (2007)

Section

Articles

License

The copyright of the articles published in Potato Journal is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

Kaushal, N., Gawande, S. J., Kaundal, P., & Garg, I. D. (2013). REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR) BASED DETECTION OF PVSO AND PVSA STRAINS BY USING DEGENERATE PRIMERS. Potato Journal, 34(1 - 2). https://epubs.icar.org.in/index.php/PotatoJ/article/view/33092