DETECTION OF POTATO SPINDLE TUBER VIROID (PSTVd) USING FLUORESCEIN LABELED PROBE


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Authors

  • Yogita Verma
  • Arti Shukla
  • S. M. Paul Khurana
  • I D Garg

Abstract

A non-radioactive detection protocol based on fluorescein labeled cDNA probe was standardized in dot blot using gene images random prime labeling module. The protocol allows detection of hybrids upon incubation with an antifluorescein alkaline phosphatase conjugate. The signal was discernible upto a dilution of 1:256. To validate the potential of this technique, in-vitro tissue culture plantlets were screened with this probe and its sensitivity was also compared with 32P labeled probe.

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Author Biographies

  • Arti Shukla
    Central Potato Research Institute, Shimla-171 001, Himachal Pradesh, India.
  • S. M. Paul Khurana
    Rani Durgavati University, Jabalpur-482 001, Madhya Pradesh, India.
  • I D Garg
    Central Potato Research Institute, Shimla-171 001, Himachal Pradesh, India.

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Issue

Vol. 33 No. 3 - 4 (2006)

Section

Articles

License

The copyright of the articles published in Potato Journal is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

Verma, Y., Shukla, A., Paul Khurana, S. M., & Garg, I. D. (2013). DETECTION OF POTATO SPINDLE TUBER VIROID (PSTVd) USING FLUORESCEIN LABELED PROBE. Potato Journal, 33(3 - 4). https://epubs.icar.org.in/index.php/PotatoJ/article/view/33223