ISOLATION, CHARACTERIZATION AND EVALUATION OF ENTOMOPATHOGENIC FUNGI FROM BEETLES, BRAHMINA CORIACEA


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Authors

  • Anupam Sharma CSK HPKV, Palampur
  • Desh Raj Thakur HPU, Shimla, H. P.
  • Virender Kumar Chandla Central Potato Research Institute, Shimla

Keywords:

18S rRNA genes, ITSI and ITS2 sequence, 28S rRNA, White grub, Potato, Microbial pathogen

Abstract

White grub Brahmina coriacea is one of the most important pests of potato in North western hills of India. In this study, B. coriacea beetles were trapped and investigated for fungal pathogens of this pest. Three fungi were consistently isolated from the infected beetles. Based on partial sequencing of 18S rRNA, ITS1, 5.8S, ITS2 and a partial sequence of 28S rRNA, the isolates were identified as Beauveria bassiana strain CPRI16, Aspergillus flavus strain CPRI18 and Fusarium oxysporum strain CPRI15. Additionally, insecticidal activity of these isolates was determined on B. coriacea grubs, eggs and beetles by testing spore concentration of 1 × 108 spores/ml. The highest mortality of second (92.59%) and third instar grubs (81.85%) was obtained from B. bassiana after 60 days of treatment. Adults’ mortality upto 100% was obtained with all the isolates. Egg laying and percent hatching of eggs was observed least due to A. flavus. 

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Author Biographies

  • Anupam Sharma, CSK HPKV, Palampur
    Research Associate, Department of Agronomy, CSK HPKV, Palampur, HP, India
  • Desh Raj Thakur, HPU, Shimla, H. P.
    Associate Professor, Department of Biosciences HPU, Shimla, H. P.
  • Virender Kumar Chandla, Central Potato Research Institute, Shimla

    Prinicipal Scientist (Retd.), Division of Plant Protection, Central Potato Research Institute, Shimla, H. P. 

Submitted

2016-05-27

Published

2017-04-17

Issue

Vol. 43 No. 2 (2016)

Section

Articles

License

The copyright of the articles published in Potato Journal is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

Sharma, A., Thakur, D. R., & Chandla, V. K. (2017). ISOLATION, CHARACTERIZATION AND EVALUATION OF ENTOMOPATHOGENIC FUNGI FROM BEETLES, BRAHMINA CORIACEA. Potato Journal, 43(2). https://epubs.icar.org.in/index.php/PotatoJ/article/view/58714