Differential expression of induced resistance genes by Abscisic acid (ABA) against potato early blight disease
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Authors
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eman el-argawy
Plant Pathology Department , Faculty of Agriculture, Damanhour University, Damanhour, Albeheira, PO Box 22516, Egypt.
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Ibrahim Adss
Division of Genetics, Faculty of Agriculture, Damanhour University, Damanhour, Albeheira, PO Box 22516, Egypt.
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Shokry Bayoumi
Division of Genetics, Faculty of Agriculture, Damanhour University, Damanhour, Albeheira, PO Box 22516, Egypt.
Keywords:
Potato, early blight, Abscisic acid (ABA), CuZnSOD gene - WRKY gene.Abstract
Abscisic acid (ABA) enhanced disease resistance of two potato cultivars i.e., Nicola and Spunta, against early blight disease. Treatment with ABA solution (10 µM) reduced the early blight disease severity for both cultivars compared to the untreated infected plants. Differential display gene analysis for treated plants revealed three clear bands. These bands showed significant homology to well-known genes in the GenBank database and identified as superoxide dismutase (CuZnSOD), WRKY transcription factor (StWRKY) and Endoglucanase from potato genome. RT-PCR showed that the expression (activity) level of CuZnSOD and WRKY genes in were increased ABA treated plants compared to the untreated plants.
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