OSMOTIN AN ANTIFUNGAL CYTOTOXIC FROM SOLANUM TUBEROSUM: NEW INSIGHTS INTO STRUCTURAL AND ANTIFUNGAL PROPERTIES
Keywords:Antifungal alignment, homology modelling, osmotin-like proteins, procheck validation, 3D structure
AbstractPathogenesis related (PR) proteins are considered as the major sources in plantsâ€™ defence response against pathogens as well as other biotic and abiotic stresses. We present here the 3-D structure of potato (Solanum tuberosum L.) osmotin-like protein (StOLP) based on the homology modelling using structure validation programs namely PROCHECK, PROSA, VERIFY3D and WHATIF. The model revealed that StOLP protein shared 16 conserved Cys, which formed eight disulfide bonds. The 3-D structure of StOLP showed three characteristic domains showing similarity fold with the thaumatin. Domain 1 consisted of a flattened 11-Î² strands (six antiparallel Î² strands present in the front end and other five antiparallel Î² strands are located at the back) which together constituted the Î²-sandwich. Domain II consisted of an Î±-helix and three shorter domains which formed loops exposed on the molecular surface of the protein. Together with domain I, domain II formed a large cleft region on the protein surface. Domain III consisted of a small loop of 32 residues which formed a long loop consisting of two Î² sheets. This modelling effort provided structural predictions of the catalytic domain of StOLP that could be verified via experimental techniques. Findings predicted the evidence of antifungal activity for StOLP against the oomycete Phytophthora infestans causes late blight disease in potato.
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How to Cite
Singh, R., Tiwari, J. K., Rawat, S., & Chakrabarti, S. K. (2017). OSMOTIN AN ANTIFUNGAL CYTOTOXIC FROM SOLANUM TUBEROSUM: NEW INSIGHTS INTO STRUCTURAL AND ANTIFUNGAL PROPERTIES. Potato Journal, 44(1). Retrieved from https://epubs.icar.org.in/index.php/PotatoJ/article/view/72021