DEVELOPMENT OF DETECTION ASSAY FOR EARLY INFECTION OF LATE BLIGHT IN POTATO USING FLOW CYTOMETRY

Authors

  • HEMANT Balasaheb KARDILE ICAR-Central Potato Research Institute, Shimla
  • Nirmal Kant Sharma ICAR-CPRI, Shimla
  • Ankita Sharma Department of Biotechnology, YSPUHF, Nauni, Solan, Himachal Pradesh, India
  • Sanjeev Sharma ICAR-CPRI, SHIMLA
  • Vinay Bhardwaj ICAR_CPRI, Shimla
  • Swarup Kumar Chakrabarti ICAR-CPRI, Shimla

Keywords:

FACS, P. infestans, Modified HPI

Abstract

Late blight is the most devastating disease of potato caused by hemibiotrophic fungus Phytophthora infestans.
The disease has two distinct phases of its life cycle: an early asymptomatic biotrophic phase and a late necrotrophic phase.
An early biotrophic phase remains for about 72 hrs and is often difficult to detect. Here, we present the simple fluorescence
activated cell sorter (FACS) based method to detect the P. infestans population at an early stage (before necrotrophic phase)
of late blight infection using the same voltage gain settings which is generally used for DNA ploidy estimation of potato. An
early detection of P. infestans populations in leaf samples will allow the sufficient time for the curative measures.

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Published

2018-07-01

How to Cite

KARDILE, H. B., Sharma, N. K., Sharma, A., Sharma, S., Bhardwaj, V., & Chakrabarti, S. K. (2018). DEVELOPMENT OF DETECTION ASSAY FOR EARLY INFECTION OF LATE BLIGHT IN POTATO USING FLOW CYTOMETRY. Potato Journal, 45(1). Retrieved from https://epubs.icar.org.in/index.php/PotatoJ/article/view/80865

Issue

Vol. 45 No. 1 (2018)

Section

Articles