INVESTIGATING THE BEST SUITABLE NUCLEAR ISOLATION BUFFER FOR POTATO FLOW CYTOMETRY


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Authors

  • HEMANT Balasaheb KARDILE ICAR-Central Potato Research Institute, Shimla

Keywords:

DNA ploidy, 2C DNA content, modified HPI buffer

Abstract

Potato harbors great amount of diversity in terms of its ploidy. The progress of breeding programme is often
limited by the hybridization barriers confronted due to differences in ploidy. Hence, understanding the DNA ploidy is
of prime importance. The DNA ploidy can be estimated using flow cytometry. Great choices of nuclear isolation buffers
(NIB) are available for sample preparation in flow cytometry. Selecting the best suitable among them is critical and also
govern the success of experiment. Here, we tested five modified NIBs to identify the best suitable one for potato sample
preparation. Investigation reveals best suitability of modified HPI buffer for flow cytometry in potato. The same buffer
was used to estimate the genome size and ploidy of randomly selected potato samples.

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Submitted

2020-03-13

Published

2021-09-01

Issue

Vol. 47 No. 1 (2020): Potato Journal

Section

Articles

License

The copyright of the articles published in Potato Journal is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

KARDILE, H. B. (2021). INVESTIGATING THE BEST SUITABLE NUCLEAR ISOLATION BUFFER FOR POTATO FLOW CYTOMETRY. Potato Journal, 47(1). https://epubs.icar.org.in/index.php/PotatoJ/article/view/99011