DEVELOPMENT OF SSR FINGERPRINTS OF INTERSPECIFIC POTATO SOMATIC HYBRIDS


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Authors

  • Jagesh Kumar Tiwari Scientist, Division of Crop Improvement, CPRI, Shimla 171 001, HP

Keywords:

Genetic fidelity, potato somatic hybrids, SSR markers

Abstract

The objective of this study was to develop DNA fingerprints of potato progenies by SSR markers for genetic
fidelity purpose. Interspecific potato somatic hybrid progenies, developed earlier by crossing somatic hybrids with Indian
potato varieties, were used in this study. A total of 81 genotypes (progenies and parents) were analysed by two wellcharacterized
potato SSR markers (STU6SNRN and STIIKA), and developed a data matrix for genetic fidelity purpose and
other applications. High polymorphism was observed in STIIKA (PIC: 0.98) than STU6SNRN (PIC: 0.95), and also higher
number of alleles was observed in STIIKA (22) than STU6SNRN (7). In STU6SNRN, alleles size 179, 182, 190 and 200 bp
were predominant; whereas in STIKA, alleles size 195, 201, 221, 235, 242 and 245 were observed frequently in more than
50 per cent of the genotypes. Diversity analysis showed a clear distinction among the genotypes based on the Jaccard
dissimilarity coefficient by the Neighbour-joining tree method using the DARwin software. Our study suggests that SSR
fingerprints would be of practical utility to strengthen genetic fidelity of these somatic hybrids. Further, SSR fingerprints
would also be valuable resources in resolving issues related to identification of true-to-type clone during field evaluation
in multiple environments.

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Author Biography

  • Jagesh Kumar Tiwari, Scientist, Division of Crop Improvement, CPRI, Shimla 171 001, HP

    Crop Improvement

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Submitted

2020-03-18

Published

2021-09-01

Issue

Vol. 47 No. 1 (2020): Potato Journal

Section

Articles

License

The copyright of the articles published in Potato Journal is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

Tiwari, J. K. (2021). DEVELOPMENT OF SSR FINGERPRINTS OF INTERSPECIFIC POTATO SOMATIC HYBRIDS. Potato Journal, 47(1). https://epubs.icar.org.in/index.php/PotatoJ/article/view/99136