Comparative Efficacy of Gamma Interferon Assay with Comparative Intradermal Tuberculin Skin Test in Diagnosing Tuberculosis in Dairy Animals

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  • Anukirat Nanda Deptt. of Veterinary Medicine, COVS, GADVASU, Ludhiana
  • Sarnarinder Singh Randhawa Department of Veterinary Medicine, COVS, GADVASU, Ludhiana
  • Deepti Narang


bTB, CITT, Paratuberculosis


Comparative merits of Comparative intradermal test (CITT) and Gamma interferon assay (IFN-γ) were assessed in detecting bovine tuberculosis (bTB) in dairy cows and buffaloes (n=201) having clinical symptoms and history suggestive of TB. Out of the 201 animals tested, 19.4%, 10% and 14.4% were positive for TB by CITT only, IFN-γ assay only and by both the tests, respectively. This clearly demonstrated that these tests, if performed individually, would not be enough to accurately detect TB in dairy animals. Instead, these would complement each other for efficient diagnosis of TB. Amplification of IS900 gene sequence specific for Mycobacterium avium subspecies paratuberculosis revealed 229 bp band in DNA extracted from the fecal samples of 120 animals. The percentage of Johne’s disease was 12.5%.

Author Biographies

  • Anukirat Nanda, Deptt. of Veterinary Medicine, COVS, GADVASU, Ludhiana
    M.V.Sc student, Deptt. of Veterinary Medicine, COVS, GADVASU, Ludhiana
  • Deepti Narang
    Senior  Scientist, Department of Veterinary Microbiology, COVS, GADVASU, Ludhiana


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How to Cite

Comparative Efficacy of Gamma Interferon Assay with Comparative Intradermal Tuberculin Skin Test in Diagnosing Tuberculosis in Dairy Animals. (2019). ISVIB Journal Veterinary Immunology & Biotechnology, 1(2).