Genotypic and Phenotypic Correlation of Antimicrobial Resistance in Bacterial Isolates from Mastitic Milk
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Authors
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Gurleen Kour
Guru Angad Dev Veterinary and Animal Sciences University
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Mudit Chandra
Guru Angad Dev Veterinary and Animal Sciences University
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Gurpreet Kaur
Guru Angad Dev Veterinary and Animal Sciences University
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Deepti Narang
Guru Angad Dev Veterinary and Animal Sciences University
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Dhiraj Kumar Gupta
Guru Angad Dev Veterinary and Animal Sciences University
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Anil Kumar Arora
Guru Angad Dev Veterinary and Animal Sciences University
Keywords:
Mastitis, milk, PCR, antibiotic resistance genesAbstract
In the present study, milk samples from mastitic animals were collected and screened for the isolation and identification of bacterial etiological agents. Out of a total of 102 milk samples, 50 samples yielded bacterial growth. The bacteria identified were Staphylococcus (43), Streptococcus (15), E. coli (26) and Klebsiella (19). All the bacterial isolates were subjected to antibiotic sensitivity testing. Further, DNA from these bacterial isolates was isolated and subjected to PCR using genus specific primers which revealed that all the isolates were conforming to their respective genus. Also, DNA was subjected to  PCR to detect the presence of aac (3)-iv, blaSHV, aadA and tetB, tetM, erm A, mec A and blaZ, tetM, ermB, blaZ and mefA antibiotic resistance genes revealed that there was an absolute correlation between the genotypic and phenotypic results of the antibiotic resistance.
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