Expression of Cathelicidins in Bovine Tissues
Abstract views: 92 / PDF downloads: 10
Keywords:Anti microbial proteins, cathelicidins, gene expression, Jersey Red Sindhi crossbred cow
Anti microbial proteins (AMPs) are host defense molecules that constitute anÂ important part of the innate immunity in animals. This study was carried out to investigate the expression of some related AMPs belonging toÂ cathelicidins family; namely cathelicidins 1, cathelicidins 2, cathelicidins 3 and cathelicidins 4 in seven milk samples with varying somatic cell counts, udder and liverÂ tissues of Jersey Red Sindhi crossbred cows. For this, milk samples were collected and evaluated for somatic cell counts. Liver and udder tissue were also collected for studying the expression of these AMPs. The gDNA was isolated from liver tissues. Pairs of primers viz CathL1, CathL2, CathL3 and CathL4 were designed and tested initially on gDNA. Total RNA was isolated from the tissues and the milk samples followed by cDNAs preparation. Expression of cathelicidins coding genes vizcathelicidins1, cathelicidins 2 and cathelicidins 3 was found in liver tissue but not of cathelicidins 4 gene. Expression of cathelicidins 2 gene was found in udder tissue but not of cathelicidins 1, cathelicidins 3 and cathelicidins 4 genes, respectively. Expression of cathelicidins1, cathelicidins 2, cathelicidins 3 and cathelicidins 4 genes, respectively was not found in the selected milk samples of Jersey Red Sindhi crossbred cows.
Brogden K, Ackermann M, Paul B, Mcray P (2003) Antimicrobial peptides in animals and their role in host defence. Int J Antimicrob Agents 22: 465-478.
Lisowski P, Jarczak J, Krzyzewski J, Zwierzchowski L, Bagnicka E (2014)Expression patterns of Î²-defensin and cathelicidin genes in parenchyma of bovine mammary gland infected with coagulase-positive or coagulase-negative Staphylococcus spp. BMC Vet Res 10: 246-253.
Lisowski P, PierzchaÅ‚a M, Goscik J, Pareek CS, Zwierzchowski L (2008) Evaluation of reference genes for studies of gene expression in the bovine liver, kidney, pituitary, and thyroid. J Appl Genet 49: 367-372.
Malinowski E (2001) Somatic cells in milk. Medycyna Weterynaryjna 57: 13-17.
Prescott SC and Breed RS(1910) The determination of number of body cells in milk by a direct method. Am J Hyg 20: 663-664.
Reddy KV, Yedery RD, Aranha C (2004) Antimicrobial peptides: premises and promises. Int J Antimicrob Agents 24:536â€“547.
Smolenski GA, Broadhurst MK, Stelwagen K, Haigh BJ, Wheeler TT (2014) Host defense related responses in bovine milk during an experimentally induced Streptococcus uberis infection. Proteome Sci 12: 19.
Strauss WM (2001) Preparation of genomic DNA from mammalian tissue. In: Strauss WM, editor. Current Protocols in Molecular Biology Chapter 2:Unit 2.2. doi: 10.1002/0471142727.mb0202s42
Tomasinsig L, De Conti G, Skerlavaj B, Piccinini R, Mazzilli M, Este F, Tossi A, Zanetti M (2010) Broad-spectrum activity against bacterial mastitis pathogens and activation of mammary epithelial cells support a protective role of neutrophil cathelicidins in bovine mastitis. Infect Immun 78: 1781-1788.
Ulm H, Wilmes M, Shai Y, Sahl HG (2012) Antimicrobial host defensins- specific antibiotic activities and innate defense modulation. Front Immunol 3: 249.
Verbeke J, Van Poucke M, Peelman L, De Vliegher S (2015) Differential expression of CXCR1 and commonly used reference genes in bovine milk somatic cells following experimental intramammary challenge. BMC Genet 16 :40.
Whelehan CJ, Barry-Reidy A, Meade KG, Eckersall PD, Chapwanya A, Narciandi F, Lloyd AT, Oâ€™Farrelly C (2014) Characterisation and expression profile of the bovine cathelicidin gene repertoire in mammary tissue. BMC Genomics 15: 128.
Zanetti M (2004) Cathelicidins, multifunctional peptides of the innate immunity. J Leukoc Biol 75: 39â€“48.
How to Cite
LicenseThe copyright of the articles published in Veterinary Immunology & Biotechnology vests with the Indian Society for Veterinary Immunology & Biotechnology (ISVIB), who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The ISVIB has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use.
Submission of a manuscript to VIB implies that if and when the manuscript is accepted for publication, the authors agree to automatic transfer of the copyright to the publisher.