Expression of Cathelicidins in Bovine Tissues
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Keywords:Anti microbial proteins, cathelicidins, gene expression, Jersey Red Sindhi crossbred cow
Anti microbial proteins (AMPs) are host defense molecules that constitute anÂ important part of the innate immunity in animals. This study was carried out to investigate the expression of some related AMPs belonging toÂ cathelicidins family; namely cathelicidins 1, cathelicidins 2, cathelicidins 3 and cathelicidins 4 in seven milk samples with varying somatic cell counts, udder and liverÂ tissues of Jersey Red Sindhi crossbred cows. For this, milk samples were collected and evaluated for somatic cell counts. Liver and udder tissue were also collected for studying the expression of these AMPs. The gDNA was isolated from liver tissues. Pairs of primers viz CathL1, CathL2, CathL3 and CathL4 were designed and tested initially on gDNA. Total RNA was isolated from the tissues and the milk samples followed by cDNAs preparation. Expression of cathelicidins coding genes vizcathelicidins1, cathelicidins 2 and cathelicidins 3 was found in liver tissue but not of cathelicidins 4 gene. Expression of cathelicidins 2 gene was found in udder tissue but not of cathelicidins 1, cathelicidins 3 and cathelicidins 4 genes, respectively. Expression of cathelicidins1, cathelicidins 2, cathelicidins 3 and cathelicidins 4 genes, respectively was not found in the selected milk samples of Jersey Red Sindhi crossbred cows.
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