Therapeutic application of lytic bacteriophages in mastitis caused by Pseudomonas aeruginosa.
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Authors
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Lakshmi Kavitha Kommalapati
Sri Venkateswara Veterinary University, Tirupathi
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Sambasiva Rao K
Junior Research Fellow, SVVU, Tirupathi
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Srivani M
Sri Venkateswara Veterinary University, Tirupathi.
Keywords:
Pseudomonas aeruginosa – mastitis – lytic bacteriophages – therapy – miceAbstract
Mastitis by Pseudomonas aeruginosa is opportunistic and environmental in nature. The treatment often fails due to higher antibiotic resistance and many a times end with recurrence or chemical treatment of affected quarter. Hence lytic bacteriophages were studied as an alternative therapeutic approach to antibiotics. For this purpose lytic bacteriophages were isolated using double agar over lay method using prophage free culture of Pseudomonas aeruginosa. Five lytic phages showing collective 100% host range were further characterized biophysically. The phages resisted pH 4,7,9 and temperatures of 16°C, 37°C and 42°C . On in vivo therapeutic study in mice the lytic phage mixture at MOI 101 has decreased the inflammation of mammary gland effectively. The study revealed the application of lytic bacteriophages is worth as therapeutic agent and need further evaluation.
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