Differential expression study of DRB1 gene transcripts in Haranali sheep exposed to Haemonchus contortus


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Authors

  • Anu Kumari Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar
  • Aman Kumar LUVAS , Hisar
  • Vinay Kumar Department of Animal Biotechnology LUVAS, Hisar
  • Prince Tomar HVS, Haryana
  • Manohar Lal Sangwan

Keywords:

Haemonchus contortus, faecal egg count, DRB1 gene, real time PCR

Abstract

The Major Histocompatibility class II complex plays an extremely important role in disease resistance and susceptibility of Haemonchus contortus in sheep. Gastrointestinal nematodes constitute a major cause of morbidity and mortality in grazing ruminants. This study was designed to carry out the differential expression of DRB1 gene transcript in low and high fecal egg count animals by real time PCR. Animals were selected on the basis of fecal egg count (FEC). Blood was collected from jugular vein and mRNA was extracted by trizol method. Further, RT-qPCR was done to analyze the differential expression of DRB 1 exon 2 in Low FEC and high FEC animals. The expression level of DRB1 gene transcript was found to be higher in low FEC animals as compare to high FEC animals. Sheep showing resistance to Haemonchus contortus that is having Low FEC had lower (P < 0.05) DRB1*1101 expression than susceptible animals having high FEC.

Author Biographies

  • Anu Kumari, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar

    HVS, Haryan

  • Aman Kumar, LUVAS , Hisar

    Scientist, Department of Animal Biotechnology

    LUVAS,  Hisar

  • Vinay Kumar, Department of Animal Biotechnology LUVAS, Hisar
    PhD scholar,Department of Animal Biotechnology
  • Prince Tomar, HVS, Haryana
    HVS
  • Manohar Lal Sangwan
    Professor (Retd)

References

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Submitted

2018-04-06

Published

2019-05-22

Issue

Vol. 1 No. 1 (2018): ISVIB Journal Veterinary Immunology & Biotechnology

Section

Articles

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How to Cite

Kumari, A., Kumar, A., Kumar, V., Tomar, P., & Sangwan, M. L. (2019). Differential expression study of DRB1 gene transcripts in Haranali sheep exposed to Haemonchus contortus. ISVIB Journal Veterinary Immunology & Biotechnology, 1(1). https://epubs.icar.org.in/index.php/VIB/article/view/78601