A High Performance Liquid Chromatographic Method for Quantitative Determination of Tetracyclines and its Epimers in Shrimp Muscle
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Keywords:
HPLC method, tetracycline, oxytetracy- cline, chlortetracycline, tetracycline epimers, shrimp muscleAbstract
A method for determination of tetracycline, oxytet- racycline, chlortetracycline, 4-epitetracycline, 4- epioxytetracycline and 4-epichlortetracycline in shrimp muscle tissue by high performance liquid chromatographic method (HPLC) with a UV detector is described. The method involves extrac- tion of the analytes by McIlvaine buffer, clean up by C18 SPE cartridge and analysis by HPLC and UV detection. A good separation of all the six analytes was achieved and good recovery (58 to 89%), repeatability and within laboratory reproducibility were possible with this method.Downloads
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Copyright rests with the Society of Fisheries Technologists (India).How to Cite
Thomas, S., Priya, L., Prasanth, K. P., & Viswanathan Nair, P. G. (2013). A High Performance Liquid Chromatographic Method for Quantitative Determination of Tetracyclines and its Epimers in Shrimp Muscle. Fishery Technology, 50(1). https://epubs.icar.org.in/index.php/FT/article/view/26606