Cardio-protective effect of Thiamine and Pyridoxine loaded Vanillic acid grafted Chitosan micro-particles against Isoproterenol-induced myocardial Infarction in animal model

Abstract

In the present study, the cardio-protective effect of dietary supplementation of thiamine and pyridoxine loaded vanillic acid grafted chitosan microparticles (TPVGC) on isoproterenol induced myocardial infarction in Wistar strain albino rats were studied. Animals were distributed into four experimental groups which were fed at graded level of levels TPVGC for 45 days viz., Control (C) (basal feed + 0% TPVGC); T1 (basal feed + 0.8% TPVGC); T2 (basal feed + 1.6% TPVGC); T3 (basal feed + 2.4% TPVGC) for experimental period of 45 days. The possible role of dietary TPVGC on cardioprotective effect was assessed in terms of Serum LDH, IgG, Homocysteine, Serum Myglobin, troponin–T, CPK-MB, SGOT, S-GPT, and other metabolic enzyme assays. All the assessed parameters were found to have significant difference between the control and treatment groups. The biochemical markers such as troponin– T, Serum LDH, CPK-MB, S-GOT, S-GPT, IgG, Homocysteine, Serum Myglobin exhibited significant (p<0.05) difference between the control and treatment groups. Moreover, metabolic and antioxidant enzyme activity in the tissue levels also showed significant (p<0.05) difference between the control and treatment groups. Results from the present study, revealed that dietary supplementation of thiamine and pyridoxine loaded vanillic acid grafted chitosan microparticles at a minimum level of 1.6% have protective effect on experimental myocardial infarction induced by isoprenaline in rats.