High-Throughput Approach for Evaluating Antibacterial Activity of Fish Epidermal Mucus
Antibacterial Activity of Fish Epidermal Mucus
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Authors
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Visnuvinayagam Sivam
ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Willingdon Island, Cochin-29
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Subie Thankam Cheriyan
School of Applied Sciences, Centre for Professional and Advanced Studies, Pathanamthitta, Kerala
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Chandran A.
School of Applied Sciences, Centre for Professional and Advanced Studies, Pathanamthitta, Kerala
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Ezhil Nilavan S.
ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Willingdon Island, Cochin-29
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Murugadas V.
ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Willingdon Island, Cochin-29
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Toms C. Joseph
ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Willingdon Island, Cochin-29
Keywords:
Fish mucus, fish epidermal mucus, antimicrobial activity, antimicrobial peptideAbstract
Fish epidermal mucus (FEM) is a source of antimicrobial peptides (AMP) that play an important role in the innate immunity of fish. Currently, there are no simple and quick protocols for screening FEM for antimicrobial activity. The present work describes the optimization of a modified optical density reduction (MODR) method to screen the FEM for antibacterial activity against various bacteria within 8 h. Reduction in optical density values in the bacteria compared with positive control suggest FEM sample exhibits antibacterial activity. The MODR approach is a low-cost, rapid and solvent-free method, and can be used to screen the FEM for potential antibacterial activity.
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Submitted
2024-06-14
Published
2025-01-31
Issue
Section
Research Note
License
Copyright rests with the Society of Fisheries Technologists (India).How to Cite
Sivam, V., Cheriyan, S. T., Chandran, A., Nilavan, S. E., Murugadas, V., & Joseph, T. C. (2025). High-Throughput Approach for Evaluating Antibacterial Activity of Fish Epidermal Mucus: Antibacterial Activity of Fish Epidermal Mucus. Fishery Technology, 62(1). https://doi.org/10.56093/ft.v62i1.152775
