Comparison of Collagen Extracted from Skin of Doublespotted Queenfish and Malabar Grouper

G S Hema; K Shyni; Suseela Mathew; George Ninan; C G Joshy

doi:10.56093/ft.v51i2.39990

Authors

G S Hema Central Institute of Fisheries Technology, P.O. Matsyapuri, Cochin - 682 029, India
K Shyni Central Institute of Fisheries Technology, P.O. Matsyapuri, Cochin - 682 029, India
Suseela Mathew Central Institute of Fisheries Technology, P.O. Matsyapuri, Cochin - 682 029, India
George Ninan Central Institute of Fisheries Technology, P.O. Matsyapuri, Cochin - 682 029, India
C G Joshy Central Institute of Fisheries Technology, P.O. Matsyapuri, Cochin - 682 029, India

https://doi.org/10.56093/ft.v51i2.39990

Keywords:

Acid soluble collagen, pepsin digestible collagen, yield, type I collagen

Abstract

Acid soluble collagen (ASC) and pepsin digestible collagen (PDC) from the skin of double-spotted queenfish (Scomberoides lysan) and Malabar grouper (Epinephelus malabaricus), were isolated and characterized. On wet weight basis, the yields of ASC and PDC from queen fish and grouper were 7.82, 3.92, 12.5 and 6.49% respectively. Amino acid analysis revealed that they contained glycine as a major amino acid with high contents of alanine, proline and hydroxyproline. Based on sodium dodecyl sulfate polyacrylamide gel electrophoretic patterns and subunit compositions, all were identified to be type 1 collagens when compared with calf skin type 1 collagen. a1, a2 and b chains were the major components of the presently isolated collagens. While comparing these two species, queen fish skin had good yield of collagen which could be served as an alternative source of collagen for different applications.