STUDIES ON FISH liPIDS II. FATTY ACID COMPOSITION QF LIPIDS OF Oil SARDINE (SARDINELLA LONGICEPS)

Abstract

Gas-liquid chromatography has been employed for the qualitative
and quantitative analysis of the component fatty acids in lipids
of oil sardine (Sardinella longiceps). Phospholipids and triglycerides
of the lipids were previously separated by column chroma·
tography before they were converted into the methyl esters of
the fatty acids. The predominant acids present in the depot fat of
thefishhavebeenfoundtobeC 14:o = 8.13%, C16:o = 27.9%,
C18:o = 3.8%,C18:1 = 15.4%. C20 :5 = 10.6% andC22 :6 =8.8%.
Apart from the above acids the .distribution of minor acids
belonging to cl81 c2 0 and c2 2 groups have also been worked
out. The separated phospholipid fraction contained more than 70%
polyunsaturated acids of which the important constituents were
docosahexaenoic (C2 2 :6 =28%) and eicosapentaenoic (C2 o:s· =
10.6%). A marked reduction was found in the amounts of
polyunsaturated acids in triglycerides, their total amount registering
about 20 percent. This fraction recorded about 48 per cent of C16
acids of which palmitic and palmitoleic acids amounted to 25.8
per cent and 19.1 per cent respectively. Occurrence of odd
numbered fatty acids c15 and cl 7 has also been noted in the
phospholipid and. composite samples of the fish.