Endophytic bacteria improve mesorhizobial nodulation, plant growth and yield in chickpea (Cicer arietinum)

KARIVARADHARAJAN SWARNALAKSHMI; SUSHMITA RAJKHOWA; VIJAY POONIYA; KANNEPALLI ANNAPURNA

doi:10.56093/ijas.v91i7.115027

Authors

KARIVARADHARAJAN SWARNALAKSHMI ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
SUSHMITA RAJKHOWA ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
VIJAY POONIYA ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
KANNEPALLI ANNAPURNA ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India

https://doi.org/10.56093/ijas.v91i7.115027

Keywords:

Chickpea, Endophytic bacteria, Mesorhizobium, Nodulation, Productivity

Abstract

The aim of our study was to determine the symbiotic effectiveness of Mesorhizobium ciceri and plant growth promotion of endophytic bacteria in field-grown chickpea during 2016-17 and 2017-18. Co-inoculation of M. ciceri along with various endophytes has significantly improved soil dehydrogenase activity in chickpea rhizosphere. The combined inoculation of M. ciceri with nodule endophytes, viz. Bacillus cereus (25.7-51.9%) and Bacillus aerophilus (18.6-27.8%) showed higher nodule weight than uninoculated control which is at par with inoculation of M. ciceri with root endophyte (Pseudomonas flourescens). Co-inoculation of M. ciceri + B. aerophilus increased chickpea growth (13.7-21.5%) at 50% flowering stage and grain yield (15.6-18.2%) at harvest stage which is at par with the treatment with P. flourescens (17.5-20.1%). A positive correlation was observed between nodule dry weight with growth and yield of chickpea in the second year. Co-inoculation of endophytic bacteria has improved the symbiotic efficiency, growth and productivity of chickpea through synergistic interaction with Mesorhizobium.

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References

Ahmad F, Ahmad I and Khan M S. 2008. Screening of free-living rhiozospheric bacteria for their multiple plant growth promoting activities. Microbiological Research 163(2): 173–81. DOI: https://doi.org/10.1016/j.micres.2006.04.001

Ahmad M, Naseer, I, Hussain A, Mumtaz M Z, Mustafa A, Hilger T H, Zahir Z A and Minggang X. 2019. Appraising endophyte–plant symbiosis for improved growth, nodulation, nitrogen fixation and abiotic stress tolerance: An experimental investigation with chickpea (Cicer arietinum L.). Agronomy 9(10): 621. DOI: https://doi.org/10.3390/agronomy9100621

Ali S, Charles T C and Glick B R. 2017. Endophytic phytohormones and their role in plant growth promotion. Functional Improvement of the Plant Microbiome, pp 89–105. Doty S L (Ed), Springer International Publishing. DOI: https://doi.org/10.1007/978-3-319-65897-1_6

Aparna K, Rao D L N and Manna M C. 2014. Microbial inoculation of chickpea (Cicer arietinum L.) enhances rhizosphere effects on soil biological quality. Agrochimica 58(2): 114–25.

Casida L E. 1977. Microbial metabolic activity in soil as measured by dehydrogenase determinations. Applied Environmental Microbiology 34(6): 630–36. DOI: https://doi.org/10.1128/aem.34.6.630-636.1977

Dudeja S S, Giri R, Saini R, Suneja Madan P and Kothe E. 2012. Interaction of endophytic microbes with legumes. Journal of Basic Microbiology 52(3): 248–60. DOI: https://doi.org/10.1002/jobm.201100063

Gopalakrishnan S, Srinivas V, Alekhya G, Prakash B, Kudapa H, Rathore A and Varshney R K. 2015. The extent of grain yield and plant growth enhancement by plant growth-promoting broad-spectrum Streptomyces sp. in chickpea. Springer Plus 4(1): 31. DOI: https://doi.org/10.1186/s40064-015-0811-3

Kan F L, Chen Z Y, Wang E T, Tian C F, Sui X H and Chen W X. 2007. Characterization of symbiotic and endophytic bacteria isolated from root nodules of herbaceous legumes cultivated in Qinghai-Tibet plateau and in other zones of China. Archives of Microbiology 188: 103–15. DOI: https://doi.org/10.1007/s00203-007-0211-3

Korir H, Mungai NW, Thuita M, Hamba Y and Masso C. 2017. Co-inoculation effect of rhizobia and plant growth promoting rhizobacteria on common bean growth in a low phosphorus soil. Frontiers in Plant Science 8: 141. DOI: https://doi.org/10.3389/fpls.2017.00141

Li J H, Wang E T, Chena W F and Chena W X. 2008. Genetic diversity and potential for promotion of plant growth detected in nodule endophytic bacteria of soybean cultivated in Heilongjiang province of China. Soil Biology and Biochemistry 40: 238–46. DOI: https://doi.org/10.1016/j.soilbio.2007.08.014

Liu B, Qiao H, Huang L, Buchenauer H, Han Q, Kang Z and Gong Y. 2009. Biological control of take-all in wheat by endophytic Bacillus subtilis E1R-j and potential mode of action. Biological Control 49: 277–85. DOI: https://doi.org/10.1016/j.biocontrol.2009.02.007

Nour S M, Fernandez M P, Normand P and Cleyet-Marel J C. 1994. Rhizobium ciceri sp. nov., consisting of strains that nodulate chickpeas (Cicer arietinum L.). International Journal of Systemic Bacteriology 44: 511–22. DOI: https://doi.org/10.1099/00207713-44-3-511

Olsen S R. 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. US Department of Agriculture Circular 939: 1–19.

Peoples M B, Ladha J K and Herridge D F. 1995. Enhancing legume N2 fixation through plant and soil management. Plant and Soil 174: 83–101. DOI: https://doi.org/10.1007/978-94-011-0055-7_4

Rangeshwaran R, Raj J and Kumar P S. 2008. Identification of endophytic bacteria in chickpea (Cicer arietinum L.) and their effect on plant growth. Journal of Biological Control 22(1): 13–23.

Rosenblueth M and Martinez-Romero E. 2006. Bacterial endophytes and their interactions with hosts. Molecular Plant- Microbe Interactions 19(8): 827–37. DOI: https://doi.org/10.1094/MPMI-19-0827

Saini R, Kumar V, Dudeja S S and Pathak D V. 2015. Beneficial effects of inoculation of endophytic bacterial isolates from roots and nodules in chickpea. International Journal of Current Microbiology and Applied Science 4(10): 207–21.

Soe K M, Bhromsiri A, Karladee D and Yamakawa T. 2012.Effects of endophytic actinomycetes and Bradyrhizobium japonicum strains on growth, nodulation, nitrogen fixation and seed weight of different soybean varieties. Soil Science and Plant Nutrition 58(3): 319–25. DOI: https://doi.org/10.1080/00380768.2012.682044

Subbiah B V and Asija G L. 1956. A rapid procedure for assessment of available nitrogen in soils. Current Science 31: 196–260.

Swarnalakshmi K, Pooniya V and Paul S. 2017. Synergistic interaction of Piriformospora indica and microbial inoculants on symbiotic potential, plant nutrition and productivity of chickpea (Cicer arietinum). Indian Journal of Agronomy 62(4): 481–88.

Swarnalakshmi K, Rajkhowa S, Senthilkumar M and Dhar D W. 2019. Influence of endophytic bacteria on growth promotion and protection against diseases in associated plants. Microbial Interventions in Agriculture and Environment, Vol. 3, pp 263–87. Singh D P and Prabha R (Eds). Springer Nature Singapore Pte Ltd, Singapore. DOI: https://doi.org/10.1007/978-981-32-9084-6_12

Swarnalakshmi K, Yadav V, Tyagi D, Dhar D W, Kannepalli A and Kumar S. 2020. Significance of plant growth promoting rhizobacteria in grain legumes: growth promotion and crop production. Plants 9(11): 1596. DOI: https://doi.org/10.3390/plants9111596

Valverde A, Burgos A, Fiscella T, Rivas R, Velaz-quez E, Rodriguez-Barrueco C, Cervantes E, Chamber M and Igual J M. 2006. Differential effects of coinoculations with Pseudomonas jessenii PS06 (a phosphate-solubilizingbacteria) and Mesorhizobium ciceri C-2/2 strains on the growth and seed yield of chickpea under greenhouse and field conditions. Plant and Soil 287: 43–50. DOI: https://doi.org/10.1007/s11104-006-9057-8

Verma J P, Yadav J, Tiwari K N and Kumar A. 2013. Effect of indigenous Mesorhizobium spp. and plant growth promoting rhizobacteria on yields and nutrients uptake of chickpea (Cicer arietinum L.) under sustainable agriculture. Ecological Engineering 51: 282–86. DOI: https://doi.org/10.1016/j.ecoleng.2012.12.022

Walkley A and Black I A. 1934. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic soil titration method. Soil Science 37(1): 29–38. DOI: https://doi.org/10.1097/00010694-193401000-00003

Wani P A, Khan M S and Zaidi A. 2007. Synergistic effects of the inoculation with nitrogen-fixing and phosphate solubilizing rhizobacteria on the performance of field grown chickpea. Journal of Plant Nutrition and Soil Science 170(2): 283–28. DOI: https://doi.org/10.1002/jpln.200620602

Wolinska A and Stępniewska Z. 2012. Dehydrogenase activity in the soil environment. Dehydrogenases: 183–210. DOI: https://doi.org/10.5772/48294