SSR marker based molecular diversity analysis of soybean genotypes having differential response to yellow mosaic disease

Aravind T; K.P SINGH; KUMARI SURBHI; POOJA BHATT; SHIVANI ARAVIND; HIMANI JEENA

doi:10.56093/ijas.v96i7.138262

Authors

Aravind T G. B. Pant University of Agriculture and Technology
K.P SINGH Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India – 263145
KUMARI SURBHI Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India – 263145
POOJA BHATT Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India – 263145
SHIVANI ARAVIND Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India – 263145
HIMANI JEENA Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India – 263145

https://doi.org/10.56093/ijas.v96i7.138262

Keywords:

Yellow mosaic, Soybean, Genotype, SSR Markers, Polymorphism

Abstract

Yellow mosaic disease (YMD) is one of the major constraints to soybean cultivation especially in South East Asian countries. The advanced molecular marker-based strategies enhance the accuracy, reduce time and provide stable sources of resistance for the development of virus resistant varieties. Hence, the present investigation was aimed at identification of SSR markers associated with YMD resistance in soybean. In the field screening of 43 genotypes for three consecutive years, 16 genotypes were found highly resistant with coefficient of infection (CI) ranging from 0-4 whereas two genotypes were found highly susceptible (CI >70). Eight genotypes were found resistant, five moderately resistant, eight moderately susceptible and four were susceptible. A total of 26 alleles were amplified using 12 SSR markers with an average of 2.16 alleles per locus indicating a narrow genetic base of the genotypes. Among the markers, Satt 180, Satt 301, Satt 685, Sat 460, Satt 464 and GMHSP179 were polymorphic and had PIC values ranging from 0.12 (Satt 464) to 0.78 (Satt 180). Dendrogram based on the SSR marker profile of Satt 301, Satt 180, Satt 685 and GMHSP179 clearly differentiated the resistant and susceptible genotypes of soybean into separate clusters. Hence, these four markers can be successfully exploited for marker assisted selection of YMD resistant soybean genotypes

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