Isolation, Selection and Characterization of Salt and Temperature Tolerant Plant Growth-Promoting Rhizobacteria for Enhancing Tomato Plant Growth in Saline Environments: Isolation, Selection and Characterization of Salt and Temperature Tolerant Plant Growth-Promoting Rhizobacteria for Enhancing Tomato Plant Growth in Saline Environments

SATYAM; A.M Navale; S.V Kolase

doi:10.56093/ijas.v96i7.144619

Authors

SATYAM MAHATMA PHULE KRISHI VIDYAPEETH RAHURI
A.M Navale
S.V Kolase

https://doi.org/10.56093/ijas.v96i7.144619

Keywords:

Isolation, Selection, characterization, plant growth promoting rhizobacteria, salt and temperature tolerant

Abstract

The present study was focused on isolation, selection and characterization of salt and temperature tolerant plant growth promoting rhizobacteria from rhizosphere soil of tomato. A total of 46 isolates were developed on Ashby's Mannitol agar media in the laboratory of department of Agricultural Microbiology, MPKV, Rahuri, Maharashtra during year 2020-2023. The medium was supplied with various NaCl concentrations, MgCl₂ concentrations, and pH levels to examine the tolerance to salt and temperature. All the forty six isolates showed promising salt tolerance. Out of forty six isolates, nine isolates were extremely salt tolerant (more than 5.4 % salt tolerance limit). Two isolate were tolerant to NaCl concentration (2.10- 3.6 % salt). Nine isolates were moderately tolerant to NaCl concentration (0.09-1.50 % salt). Twenty six isolates were moderately sensitive to NaCl concentration (0.15-0.60 % salt). On the basis of the salt tolerance limit, rhizobacteria that promote plant growth in salt and temperature-tolerant environments were categorized. Out of forty six isolates, eight isolates showed growth at MgCl₂ concentration of 4.0 %, two isolates showed growth at 3.0 % MgCl₂ concentration, thirteen isolates showed growth at 2.0 % MgCl₂, fifteen isolates showed growth at 1.0 % MgCl₂ and eight isolates showed growth on 0.5 % MgCl₂ concentration. Out of the 46 isolates, eight showed growth at pH of 9.0, whereas the other 38 showed development at pH of 7.0. The plant growth promoting activity check was positive for 4 isolates (Pseudomonas furukawaii STT-A8, Achromobacter sp. STTA-12, Agrobacterium pusense STT-A24, and Agrobacterium pusense STT-A39) out of the forty six total isolates. Pseudomonas furukawaii STT-A8 (NCBI Accession no-OR432529), Achromobacter sp. STT-A12 (NCBI Accession no-OR432558) Shown temperature tolerance limit at 60⁰c and Agrobacterium pusense STT-A24 (NCBI Accession no-OR428230) and Agrobacterium pusense STT-A39 (NCBI Accession no-OR428223) shown temperature tolerance limit at 50⁰c. The experiment was done to characterize salt and temperature tolerant PGPR that would be a crucial inoculum to enhance tomato plant growth and development in a salty environment.

Keywords: Isolation, selection, characterization, salt and temperature tolerant, plant growth promoting rhizobacteria

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