Isolation, selection and characterisation of salt and temperature tolerant plant growth promoting rhizobacteria in tomato (Solanum lycopersicum) under saline environments
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Authors
SATYAM
Agricultural Technology Management Agency, Panapur, Saran, Bihar, India
Keywords:
Nutrient management, NaCl concentration, PGPR, Plant growthAbstract
The present study was focused on isolation, selection and characterisation of salt- and temperature-tolerant plant growth promoting rhizobacteria (PGPR) from rhizosphere soil of tomato (Solanum lycopersicum L.). A total of 46 isolates were developed during 2020–2023 at Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra on Ashby's Mannitol agar media. The medium was supplied with various NaCl concentrations, MgCl2 concentrations, and pH levels to examine the tolerance to salt and temperature. All the 46 isolates showed promising salt tolerance. Out of these isolates, nine isolates were extremely salt tolerant, two isolates were tolerant, nine isolates were moderately tolerant while 26 isolates were moderately sensitive to NaCl concentration. On the basis of the salt tolerance limit, rhizobacteria that promote plant growth in salt- and temperature-tolerant environments were categorised. Out of 46 isolates, eight isolates showed growth at MgCl2 concentration of 4.0%, two isolates at 3.0%, 13 isolates at 2.0% MgCl2, 15 isolates at 1.0% and eight isolates at 0.5% MgCl2. In case of pH, eight showed growth at pH of 9.0, whereas the other 38 showed development at pH of 7.0. The plant growth promoting activity check was positive for four isolates (Pseudomonas furukawaii STT-A8, Achromobacter spp. STTA-12, Agrobacterium pusense STT-A24, and A. pusense STT-A39). P. furukawaii STT-A8 and Achromobacter spp. STT-A12 showed temperature tolerance up to 60oC while pusense STT-A24 and A. pusense STT-A39 showed temperature tolerance up to 50oC. The experiment was done to characterise salt- and temperature-tolerant PGPR that would be a crucial inoculum to enhance tomato plant growth and development in saline environment.
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