Characterisation and Identification of Dark Septate Endophytic Fungi isolated from the roots of Aloe barbadensis Mill. and its application

SHINJAN DEY; DEBAPRIYA  CHOUDHURY; CHANDRAMA MUKHERJEE; SIKHA  DUTTA

doi:10.56093/ijas.v96i9.147073

Authors

SHINJAN DEY THE UNIVERSITY OF BURDWAN
DEBAPRIYA CHOUDHURY THE UNIVERSITY OF BURDWAN
CHANDRAMA MUKHERJEE THE UNIVERSITY OF BURDWAN
PROF. SIKHA DUTTA THE UNIVERSITY OF BURDWAN

https://doi.org/10.56093/ijas.v96i9.147073

Keywords:

Aloe barbadensis Mill., Dark Septate Endophytes

Abstract

Scientists are now-a-days prefer to use biofertilizers over chemical fertilizers because of their non-toxic and organic matter content. Dark septate endophytes (DSE) fungi exhibit positive effects on plant growth and nutrient uptake of host plants. Aloe barbadensis Mill. is one of the most important medicinal plants considered worldwide. Their leaves contain some important secondary metabolites like Aloin and Aloe emodin which possesses great importance in pharmaceutical industries for their medicinal properties. Thus, considering the beneficial role of DSE fungi and the growing demands of biofertilizers for sustainable agriculture, our present study was carried out during 2021-2023 at the Department of Botany, The University of Burdwan, West Bengal to isolate and characterize the DSE fungi from the roots of Aloe barbadensis Mill. After isolation, four DSE fungi were selected based on the production of clear halo zone and considered for their plant growth promoting traits. The molecular identification of the two best fungal strains was done by 18s r-DNA sequencing and were identified as Fusarium sp. and Aspergillus proliferans. Four experimental sets were prepared to study the effects of these fungal strains on the morphological growth of the plants as well as the Aloin and Aloe-emodin content in the leaves. After application of these fungal strains to the plant, significant augmentation in morphological growth and also enhanced Aloin and Aloe emodin content in treated leaves were noticed compared to the untreated control set, and thus these fungal strains might be considered as a potent biofertilizer in the near future.

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References

Addy S K, Goodman S K. 1972. Polyphenol oxidase and peroxidase activity in apple leaves inoculated with a virulent or an avirulent strain of Erwinia amylovora. Indian phytopathology 25: 575-579.

Aebi H. 1984. Catalase. In: Packer, L. ed. Methods in enzymol. Academic press, Orlando., 105: 121-126.

Arbab S, Ullah H, Weiwei W, Wei X, Ahmad S U, Wu L and Zhang J. 2021. Comparative study of antimicrobial action of Aloe vera and antibiotics against different bacterial isolates from skin infection. Vet Med Sci 7(5):2061–7.

Arnon DI. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24(1):1–15.

Dey S, Sarkar A, Dutta S. 2019. Characterisation of plant growth promoting traits and stress tolerant properties of Aspergillus terreus (MCC 1819) isolated from the rhizosphere of Aloe barbadensis Mill. and its application. Journal of Pharmacognosy and Phytochemistry 8(6):2303-2310.

Giannopolitis C N, Ries S K. 1977. Superoxide dismutases occurrence in higher plants. Plant physiology 59:309-314.

Gupta M, Kiran S, Gulati A, Singh B and Tewaria R. 2012. Isolation and identification of phosphate solubilizing bacteria able to enhance the growth and aloin-A biosynthesis of Aloe barbadensis Miller. Microbiological Research 167 (6): 358–63.

He C, Wang W and Hou J. 2019. Characterization of Dark Septate Endophytic Fungi and Improve the Performance of Liquorice under Organic Residue Treatment. Frontiers in Microbiology 10:1364.

Hou L, Yu J, Zhao L and He X. 2020. Dark Septate Endophytes Improve the Growth and the Tolerance of Medicago sativa and Ammopiptanthus mongolicus Under Cadmium Stress. Frontiers in Microbiology 10:3061.

Kaparakou E H, Kanakis C D, Gerogianni M, Maniati M, Vekrellis K, Skotti E and Tarantilis PA. 2021. Quantitative determination of aloin, antioxidant activity, and toxicity of Aloe vera leaf gel products from Greece. Journal of the Science and Food Agriculture 101(2):414–23.

Karamian R, Asadbegy M. 2016. Antioxidant Activity, Total Phenolic and Flavonoid Contents of three Onobrychis species from Iran. Pharmaceutical Sciences 22: 112-119.

Khan M I, Bashir N, Pandith S A, Shahzad A, Barvkar V T, Pable A A, Shah M A and Reshi Z A. 2023. Co-occurrence of two ascomycete endophytes as the specialized metabolite production partners in Rheum spiciforme Royle. Symbiosis 90:1–16.

Khan N, Bano A M D and Babar A. 2019. Impacts of plant growth promoters and plant growth regulators on rainfed agriculture. PLoS ONE 15(4): e0231426

Kumar S, Diksha S S, Kumar R. 2022. Biofertilizers: an ecofriendly technology for nutrient recycling and environmental sustainability. Current Research in Microbial Sciences 3: 100094.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35:1547-1549.

Mandyam K and Jumpponen A. 2005. Seeking the elusive function of the root-colonising dark septate endophytic fungi. Studies in Mycology 53: 173–89.

Mitra S S, Ghorai M, Nandy S, Mukherjee N, Kumar M, Radha, Ghosh A, Jha N K, Proćków J and Dey A. 2022. Barbaloin: an amazing chemical from the ‘wonder plant’with multidimensional pharmacological attributes. Archives of Pharmacology 395(12):1525-36.

Okamura N, Asai M, Hine N, Yagi A. 1996. High-performance liquid chromatographic determination of phenolic compounds in Aloe species. Journal of Chromatography A 746:225-231.

Patel K and Patel D K. 2013 Medicinal importance, pharmacological activities, and analytical aspects of aloin: A concise report. Journal of Acute Disease 262-9.

Saitou N, Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.

Sakulpanich A and Gritsanapan W. 2008. Extraction method for high content of anthraquinones from Cassia fistula pods. Journal of Health Research 22: 167-72.

Sambrook J, Fritsch E F, Maniatis T. 1989. Molecular Cloning a Laboratory Manual. New York: Cold Spring Harbor Laboratory Press.

Sarkar A and Dutta S. 2016. Isolation and Identification of Some Dark Septate Endophytic Fungi (DSE) from the Roots of the Ethno-Medicinal Plant Plumbago zeylanica L. International Journal of Current Microbiology and Applied Science 5(10): 698-707.

Sharma P, Kharkwal A C, Abdin M Z. 2014. Piriformospora indica improves micropropagation, growth and phytochemical content of Aloe vera L. plants. Symbiosis 64:11-23.

Silva C F, Vitorino L C, Oliveira I G, Oliveira T C, Resende E C, Pereira P S and Souchie E L. 2022. Endophytic bacteria promote growth and increase the aloin content of Aloe vera. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 21 (5): 607 – 619.

Sun R T, Feng X C, Zhang Z Z, Zhou N, Feng H D, Liu Y M, Hashem A, Arjani A B F, Abd_Allah E F and Wu Q S. 2022. Root Endophytic Fungi Regulate Changes in Sugar and Medicinal Compositions of Polygonum cuspidatum. Front. Plant Sci 13:818909.

Tamura K, Nei M, Kumar S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101:11030-11035.

Thakur D, Kaur M and Mishra A. 2017. Isolation and screening of plant growth promoting Bacillus spp. and Pseudomonas spp. and their effect on growth, rhizospheric population and phosphorous concentration of Aloe vera. Journal of Medicinal Plant Sciences 5(1): 187-92.

Verma S K, Sahu P K, Kumar K, Pal G, Gond S K, Kharwar R N and White J F. 2021. Endophyte roles in nutrient acquisition, root system architecture development and oxidative stress tolerance. Applied Microbiology 131(5): 2161–77.

Zahra W, Rai S N, Birla H, Singh S S, Rathore A S, Dilnashin H, Keswani C and Singh S P. 2020. Economic Importance of Medicinal Plants in Asian Countries. Bioeconomy for sustainable development 359-378.