Synergistic insecticidal efficacy of Beauveria bassiana with silver nanoparticles against Bihar hairy caterpillar, Spilarctia obliqua (Walker) infesting Green gram (Vigna radiata)
14
Authors
-
SOURABH MAHESHWARI
GOVIND BALLABH PANT UNIVERSITY OF AGRICULTURE & TECHNOLOGY, PANTNAGAR
Abstract
An experiment was conducted to evaluate insecticidal efficacy of entomopathogenic fungus, Beauveria bassiana alone and in combination with biosynthesized silver nanoparticles (AgNPs) against Bihar hairy caterpillar, Spilarctia obliqua (Walker) infesting green gram (Vigna radiata) under laboratory and net house conditions. The study showed that all the concentrations of AgNPs were compatible up to 10 ppm for the growth of B. basssiana and the combination of AgNPs (40 ppm) and B. bassiana (6.1 x 108) showed LC50 at 11.16 ppm and LT50 at 77.31 hours under laboratory condition. This combination was found 2.85 times more toxic than AgNPs alone. Under net house conditions, this combination brought cent percent probit mortality on 8th DAT. This novel approach for pest management showed significantly appreciable results for achieving synergistic insecticidal efficacy with the combination of microbial pesticide with nanoparticles.
Downloads
References
1. Abbott, W. S. 1925. A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 18(2), 265-267. https://doi.org/10.1093/jee/18.2.265a.
2. Ahmed, S., Ahmad, M., Swami, B. L. and Ikram, S. 2016. Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. J. Radiat. Res. Appl. Sci. 9(1), 1-7. https://doi.org/10.1016/j.jrras.2015.06.006.
3. Asghar, M. S., Sarwar, Z. M., Almadiy, A. A., Shami, A., Hadi-Mohamed, R. A., Ahmed, N., Waghulade, M. S., Alam, P., Abd-Al-Galil, F. M. 2022. Toxicological Effects of Silver and Zinc Oxide Nanoparticles on the Biological and Life Table Parameters of Helicoverpa armigera (Noctuidae: Lepidoptera). Agriculture. 12(10), e1744. https://doi.org/10.3390/agriculture12101744.
4. Deb, L., Dutta, P. 2021. Antagonistic potential of Beauveria bassiana (Balsamo) Vuillemin against Pythium myriotylum causing damping off of tomato. Indian Phytopathol. 74(3), 715-728. https://doi.org/10.1007/s42360-021-00372-w.
5. Devi, G. D., Murugan, K., Selvam, C. P. 2014. Green synthesis of silver nanoparticles using Euphorbia hirta (Euphorbiaceae) leaf extract against crop pest of cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae). J. Biopest. 7(1), 54-58.
6. Dudpuri, K. K., Purwar, J. P. 2021. Combined effect of entomopathogens with biorationals against Lepidopteran insect pests of greengram. Pantnagar J. Res. 19(2), 220-226. (https://www.gbpuat.res.in/paperdetail.php?paper=1256)
7. Finney, D. J. 1971. A statistical treatment of the sigmoid response curve: Probit analysis. Cambridge University Press, London, 633 p.
8. Gillespie, A. T. 1986. Effects of entomogenous fungi on the brown planthopper of rice Nilaparvata lugens. Monograph-British Crop Protection Council (BCPC), 34: 245-251.
9. Hassan, S. A. 1989. Testing methodology and the concept of the IOBC/WPRS working group. In: ‘Paul C. Jepson (Eds.) Pesticides and non-target invertebrates’. Lavoisier, France pp 1-8.
10. Hokkanen, H. M. T., Kotiluoto, R. 1992. Bioassay of the side effects of pesticides on Beauveria bassiana and Metarhizium anisopliae: standardized sequential testing procedure. IOBC/WPRS Bull., XI (3):148-151.
11. Jaskulski, D., Jaskulska, I., Majewska, J., Radziemska, M., Bilgin, A., Brtnicky, M. 2022. Silver nanoparticles (AgNPs) in urea solution in laboratory tests and field experiments with crops and vegetables. Materials. 15(3), e870. https://doi.org/10.3390/ma15030870.
12. Joshi, V. 2022. Green synthesis of zinc oxide nanoparticles and its synergistic effect with entomopathogenic fungus, Beaveria bassiana (Balsamo) Vullemin against Spodoptera litura (Fabricius). Thesis, Master of Science, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, 18-22p.
13. Kadam, J. R., Jaichakravathy, G. 2003. Bio-efficacy of Verticillium lecanii (Zimm.) against nymphs of maconellicoccus hirsutus Green. Proc. of the state level Sem. on Pest Manag. Sustain. Agric. Parbhani, 6-7.
14. Kah, M., Hofmann, T. 2014. Nanopesticide research: current trends and future priorities. Environ. Int. 63(1), 224-235. https://doi.org/10.1016/j.envint.2013.11.015
15. Khan, M., Azhar, U., Khan, M. A. H., Yadav, K. K., Marina, M.C., Malik, N., Yadav, V.K., Khan, A. H., Islam, S., Sharma, G. K. 2021. Agro-nanotechnology as an emerging field: a novel sustainable approach for improving plant growth by reducing biotic stress. Appl. Sci. 11(5), 2282-2289. https://doi.org/10.3390/app11052282.
16. Mohanpuria, P., Rana, N. K., Yadav, S. K. 2008. Biosynthesis of nanoparticles: technological concepts and future applications. J. Nanopart. Res., 10(1): 507-517. https://doi.org/10.1007/s44187-022-00013-9
17. Moore, D. and Prior, C. 1996. Mycoinsecticides: IPM system in agriculture. In: ‘R.K. Upadhyay, K.G. Mukerjee and R.L. Rajak. (Eds.) Biocontrol in emerging biotechnology’. Aditya Books Private Ltd., New Delhi, pp. 25-56.
18. Moorhouse, E. R., Gillespie, A. T., Sellers, E. K., Charnley, A. K. 1992. Influence of fungicides and insecticides on the entomogenous fungus Metarhizium anisopliae: A pathogen of the vine weevil, Otiorhynchus sulcatus. Biocontrol Sci. Technol. 2(1), 49-58. https://doi.org/10.1080/09583159209355217.
19. Nagar, N., Jain, S., Kachhawah, P., Devra, V. 2016. Synthesis and characterization of silver nanoparticles via green route. Korean J. Chem. Eng. 33, 2990-2997. https://doi.org/10.1007/s11814-016-0156-9.
20. Pokhriyal, N. 2022. Studies on combined effect of Bacillus thuringiensis (Berliner) and biosynthesized ZnO nanoparticles against Spodoptera litura (Fabricius) infesting Green gram. Thesis, Master of Science, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar-263145, Uttarakhand, 17-27p.
21. Priyadarshini, A. K., Murugan, K., Panneerselvam, C., Ponarulselvam, S., Hwang, J. S., Nicoletti, M. 2012. Biolarvicidal and pupicidal potential of silver nanoparticles synthesized using Euphorbia hirta against Anopheles stephensi Liston (Diptera: Culicidae). Parasitol. Res. 111(3), 997-1006. https://doi.org/10.1007/s00436-012-2924-8
22. Purwar, J. P., Sachan, G. C. 2005. Biotoxicity of Beauveria bassiana and Metarhizium anisopliae against Spodoptera litura and Spilarctia obliqua. Ann. Plant Prot. Sci. 13(2), 360-364. (https://www.indianjournals.com/ijor.aspx?target=ijor:apps&volume=13&issue=2&article=019)
23. Purwar, J. P., Sachan, G. C. 2006. Synergistic effect of entomogenous fungi on some insecticides against Bihar hairy caterpillar Spilarctia obliqua (Lepidoptera: Arctiidae). Microbiol. Res. 161(1), 38-42. https://doi.org/10.1016/j.micres.2005.04.006
24. Rai, D., Updhyay, V., Mehra, P., Rana, M., Pandey, A. K. 2014. Potential of entomopathogenic fungi as biopesticides. Ind. J. Sci. Res. Technol. 2(5), 7-13. (https://www.researchgate.net/publication/343501421_POTENTIAL_OF_ENTOMOPATHOGENIC_FUNGI_AS_BIOPESTICIDES)
25. Shah, P. A., Pell, J. K. 2003. Entomopathogenic fungi as biological control agents. Appl. Microbial. Biotechnol. 61(5), 413-423. https://doi.org/10.1007/s00253-003-1240-8
26. Sheoran, O. P., Tonk, D. S., Kaushik, L. S, Hasija, R.C., Pannu, R. S. 1998. Statistical software for agricultural Research workers. Recent Advances in information theory, Department of Mathematical Statistics, CCS HAU, Hisar. 139-143. (http://14.139.232.166/opstat/)
27. Shukla, V. K., Pandey, S., Pandey, A. C. 2010. Green synthesis of silver nanoparticles using neem leaf (Azadirachta indica) extract. In. AIP Conf. Proc. American Institute of Physics 1276(1), 43-49. https://doi.org/10.1063/1.3504340
28. Snedecor GW, Cochran WR 1967. Statistical methods, 6th ed., Iowa State University Press, Ames, Iowa, p. 274. (https://www.cabdirect.org/cabdirect/abstract/19680103239)
29. Sundara Babu, P.C. 1999. Advanced dimension in the use of entomopathogenic fungi for pest management. In: ‘R.J. Rabindra, S. Palaniswamy, P. Karuppuchamy, K. Ramaraju, P. Philip Sridhar (Eds.) Ecology Based Pest Management’. pp 85-91.
30. Tripathy, A., Raichur, A. M., Chandrasekaran, N., Prathna, T. C., Mukherjee, A. 2010. Process variables in biomimetic synthesis of silver nanoparticles by aqueous extract of Azadirachta indica (Neem) leaves. J. Nanopart. Res. 12, 237-246. https://doi.org/10.1007/s11051-009-9602-5
31. Veerasamy, R., Xin, T. Z., Gunasagaran, S., Xiang, T. F. W., Yang, E. F. C., Jeyakumar, N., Dhanaraj, S. A. 2011. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. J. Saudi Chem.. Soc. 15(2), 113-120. https://doi.org/10.1016/j.jscs.2010.06.004
32. Yah, C. S., Simate, G. S. 2015. Nanoparticles as potential new generation broad spectrum antimicrobial agents. DARU J. Pharm. Sci., 23(1): 1-14. https://doi.org/10.1186/s40199-015-0125-6
Submitted
Published
Issue
Section
License
Copyright (c) 2026 The Indian Journal of Agricultural Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The copyright of the articles published in The Indian Journal of Agricultural Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.
How to Cite
