Exploring the pivotal impact of salt stress on survival and morphological characteristics of citrus (Citrus spp.) rootstocks

Vishajeet Singh; Akhilesh Kumar Srivastava; Om Prakash; Arbind Kumar Gupta; Balaji Vikram; Paramanand Prajapati; Abhishek Pratap

doi:10.56093/ijas.v96i9.169757

Authors

Vishvajeet Singh Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh
Akhilesh Kumar Srivastava Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh
Om Prakash Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh
Arbind Kumar Gupta Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh
Balaji Vikram Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh
Paramanand Prajapati Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh
Abhishek Pratap Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh

https://doi.org/10.56093/ijas.v96i9.169757

Keywords:

Citrus rootstock, abiotic stress, salinity, citrus morphology, survivability

Abstract

In the Bundelkhand region, salinity presents a significant challenge for citrus plant growth. A study was carried out at Banda University of Agriculture and Technology during the 2023–24 academic year to assess seven citrus rootstocks namely, Rangpur lime, Cleopatra mandarin, Rough lemon, Sour orange, NRCC RS-3, NRCC RS-4, and NRCC RS-6 under varying salinity stress levels viz., control (0.07 dS/m), 2.0 dS/m, 4.0 dS/m, and 8.0 dS/m. These levels of salinity negatively impacted all growth parameters of the plants across all rootstocks when compared to the control treatment (0.07 dS/m). Specifically, plant mortality increased, and there were reductions in plant height, stem diameter, number of leaves, number of shoots, leaf area and root length. The minimum mortality percentage at 8dS/m over control was observed in Rangpur lime, followed by Cleopatra mandarin and Sour orange. The reduction percentage in plant height, stem diameter, number of leaves, and number of shoots at 8 dS/m salinity level over the control was also noted in Rangpur lime. While the minimum reduction in leaf area (13.50%) and root length (24.71%) was recorded in Cleopatra mandarin at the salinity level of 8 dS/m over the control. Rangpur lime, Sour orange, and Cleopatra mandarin showed better salinity tolerance, while Rough lemon, NRCC RS-3, and NRCC RS-6 had a moderate response. NRCC RS-4 was less tolerant, showing reduced growth at 8 dS/m compared to the control.

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Author Biographies

Vishvajeet Singh, Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh

Ph.D. Reserach Scholar, Department of Fruit Science
Akhilesh Kumar Srivastava, Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh

Professor & Head, Department of Fruit Science
Om Prakash, Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh

Assistant Professor, Department of Fruit Science
Arbind Kumar Gupta, Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh

Assistant Professor, Department of Natural Resource Management
Balaji Vikram, Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh

Assistant Professor, Department of Post Harvest Technology
Paramanand Prajapati, Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh

Ph.D. Research Scholar, Department of Fruit Science
Abhishek Pratap, Banda University of Agriculture and Technology, Banda- 210001, Uttar Pradesh

Ph.D. Research Scholar, Department of Fruit Science

References

Abadi F S G, Mostafavi M, Eboutalebi A, Samavat S and Ebadi A. (2010). Biomass accumulation and proline content of six citrus rootstocks as influenced by long-term salinity. Research Journal of Environmental Sciences 4(2): 158–65.

Anonymous (2023). Ministry of Agriculture and Farmer’s Welfare, New Delhi. http://agricoop.nic.in/en/StatHortEst

Arbona, V., Flors, V., Jacas, J., García-Agustín, P., and Gómez-Cadenas, A. (2003). Enzymatic and non-enzymatic antioxidant responses of Carrizo citrange, a salt-sensitive citrus rootstock, to different levels of salinity. Plant and cell physiology , 44, 388-394. https://doi.org/10.1093/pcp/pcg059.

Arif, M. R., Islam, M. T., & Robin, A. H. K. (2019). Salinity Stress Alters Root Morphology and Root Hair Traits in Brassica napus. Plants, 8(7), 192. https://doi.org/10.3390/plants8070192

Ashraful Alam, Hayat Ullah, Ahmed Attia & Avishek Datta (2019) Effects of Salinity Stress on Growth, Mineral Nutrient Accumulation and Biochemical Parameters of Seedlings of Three Citrus Rootstocks. International Journal of Fruit Science, 20:4, 786-804. DOI: 10.1080/15538362.2019.1674762

Babar, S., Siddiqi, E.H., Hussain, I., Hayat Bhatti, K. and Rasheed, R. (2014). Mitigating the effects of salinity by foliar application of salicylic acid in fenugreek. Physiology Journal, pp. 1-6.

Balal, R. M., Ashraf, M. Y., Khan, M. M., Jaskani, M. J., and Ashfaq, M.. (2011). Influence of salt stress on growth and biochemical parameters of citrus rootstocks. Pakistan Journal of Botany. 43. 2135-2141.

Cramer, G.R., Läuchli, A. and Polito, V.S. (1985). Displacement of Ca2+ by Na+ from the plasmalemma of root cells: a primary response to salt stress. Plant physiology, 79(1): 207-211.

El-Shazly, S. M. (2008). Response of some citrus rootstocks to salinity. Proceedings of the International Society of Citriculture, pp. 872–80. China.

Greenway, H. and Munns, R. (1980). Mechanism of salt tolerance in nonhalophytes. Annual Review Plant Physiology l31: 149–90.

Hussain, K., Majeed, A., Nawaz, K. and Nisar, M. F. (2009). Effect of different levels of salinity on growth and ion contents of black seeds (Nigella sativa L.). Current Research Journal of Biological Sciences 1(3): 135–38.

Khan, F., Shafiq, M., Haider, M., Sami, A., Arshad, A., and Anees, M. (2023). Impact Of Various Concentrations Of Nacl On Morphological Attributes Of Different Citrus Rootstocks Under Field Conditions. Bulletin of Biological and Allied Sciences Research, 2023(1), 50. https://doi.org/10.54112/bbasr.v2023i1.50

Marathe, R. A., Murkute, A. A., Kolwadkar, J., and Deshpande, C. P. (2022). Salinity tolerance potential of Citrus (Citrus spp.) rootstock genotypes. The Indian Journal of Agricultural Sciences, 92(11), 1311–1315. https://doi.org/10.56093/ijas.v92i11.120698

Murkute, A. A., Sharma, S. and Singh, S. K. (2005). Citrus in terms of soil and water salinity: A review. Journal of Scientific and Industrial Research 64: 393–402.

Murkute, A. A., Sharma, S. and Singh, S. K. (2006). Studies on salt stress tolerance of citrus rootstock genotypes with arbuscular mycorrhizal fungi. Horticulture Science 33: 70–76.

Murkute, A. A., Sharma, S., Singh, S. K. and Patel, V. B. (2009). Response of mycorrhizal citrus rootstock plantlets to salt stress. Indian Journal Horticulture 66: 456–60.

Othman, Y. A., Hanib, M. B., Ayada, J. Y. and St. Hilare, R. (2023). Salinity level influenced morpho-physiology and nutrient uptake of young citrus rootstocks. Heliyon. 9 (2). e13336. 10.1016/j.heliyon.2023.e13336

Patil, V. K. and Bhambota. J. R. (1978). Relationship between relative growth of different rootstocks seedlings of citrus and levels of salinity in soil. Research Bulletin Marathwada Agriculture University 2: 71–74.

Şahin-Çevik, M., Çevik, B. and Coşkan, A. (2020). Identification and expression analysis of salinity-induced genes in rangpur lime (Citrus limonia). Horticultural Plant Journal 6(5): 267–76.

Shahid, M. A., Balal, R. M., Khan, N., Simon-Grao, S., Alfosea-Simon, M., Camara-Zapata, J. M., Mattson, N. S. and Garcia-Sanchez, F. (2019). Rootstocks influence the salt tolerance of Kinnow mandarin trees by altering the antioxidant defense system, osmolyte concentration, and toxic ion accumulation. Scientia Horticulturae, 250: 1–11.

Sharma, L. K., Kaushal, M., Bali, S. K. and Choudhary, O. P. (2013). Evaluation of rough lemon (Citrus jambhiri Lush.) as rootstock for salinity tolerance at seedling stage under in vitro conditions. African Journal of Biotechnology 12(44): 6267–75.

Sheoran, O. P., Tonk, D. S., Kaushik, L. S., Hasija, R. C. and Pannu, R. S. (1998). Statistical software package for agricultural research workers. Department of Mathematics Statistics, CCS Haryana Agricultural University, Hisar, Haryana. pp. 139–43.

Simpson, C. R., Nelson, S. D., Melgar, J. C., Jifon, J., Schuster, G. and Volder, A. (2015). Effects of salinity on physiological parameters of grafted and ungrafted citrus trees. Scientia Horticulturae, 197: 483–89.

Singh, N. T., and Bandopadhyay, A. K. (1996). Chemical degradation leading to salt affected soils and their management for agriculture and alternate uses. Tech. Bull. No. 17, Indian Society for Soil Science, New Delhi, India.

Stover, E., Hall, D. G., Grosser, J., Gruber, B. and Moore, G. A. (2018). Huanglongbing-related responses of ‘Valencia’ Sweet Orange on eight citrus rootstocks during greenhouse trials. Hort Technology, 28(6): 776–82.