Identification of amplified fragment length polymorphism markers associated with Karnal bunt (Neovossia indica) resistance in bread wheat


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Authors

  • SUKHWINDER SINGH
  • TEJINDER SINGH GREWAL
  • HARJIT SINGH
  • MONIKA SODHI
  • H S DHALIWAL

Keywords:

amplified fragment length polymorphism markers, Karnal bunt, marker aided selection, Neovossia indica, Triticum aestivum

Abstract

Amplified fragment length polymorphism markers were used to study polymorphism and their association with
Karnal bunt resistance genes in wheat (Triticum aestivum L. emend. Fiori & Paol.). Recombinant inbred lines developed
from a cross of a Karnal bunt susceptible 'WL 711' and a Kamal-bunt resistant line 'HD 29' were tested for
resistance to 2 Kamal bunt isolates through artificial inoculations. Study of parents and a set of recombinant inbred
lines (selected on the basis of Karnal bunt reaction) for amplified fragment length polymorphism with 6 primer combinations
were analyzed and 336 loci scored. Of these, 77 were polymorphic between the parents. Three of the polymorphic
markers were associated with Karnal bunt resistance genes.

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Author Biographies

  • SUKHWINDER SINGH
    Assistant Plant Pathologist, Panjab Agricultural University, Ludhiana 141 004

  • TEJINDER SINGH GREWAL
    Assistant Plant Pathologist, Panjab Agricultural University, Ludhiana 141 004
  • HARJIT SINGH
    Senior Geneticist, Panjab Agricultural University, Ludhiana 141 004
  • MONIKA SODHI
    Assistant Biotechnologist, Panjab Agricultural University, Ludhiana 141 004
  • H S DHALIWAL
    Director, Biotechnology Centre, Panjab Agricultural University, Ludhiana 141 004

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Issue

Vol. 69 No. 7 (1999)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Agricultural Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

SINGH, S., GREWAL, T. S., SINGH, H., SODHI, M., & DHALIWAL, H. S. (2013). Identification of amplified fragment length polymorphism markers associated with Karnal bunt (Neovossia indica) resistance in bread wheat. The Indian Journal of Agricultural Sciences, 69(7). https://epubs.icar.org.in/index.php/IJAgS/article/view/26844