CP-HSP gene transcribe in rice (Oryza sativa) under heavy metal stress

Abstract

Chloroplast small heat shock protein (CP-sHSP) genes activate in different plants when under stress. Present investigation revealed that chlorophyll content decreased in rice (Oryza sativa) when under heavy metal stress. Total chlorophyll content decreased up to 36.61% and 42.6% when rice plants were grown at 20mM cadmium and nickel heavy metals, respectively. However, copper stress did not drop the chlorophyll content at threshold level. Transcriptome analysis shows that CP-HSPs gene transcribes in heavy metals stressed plants. Based on these observations, CP-HSPs gene was polymerized using standard primer and sequenced. The sequence alignment with CP-HSPs from gene bank divulges that Oryza sativa CP-HSP gene has 100% similarity with Arabidopsis CP-HSP 21 gene. While others; such as from other monocots and dicots, have varying resemblance. Translational sequence was proposed based on gene sequence. Phylogenetic analysis based on gene and protein sequences demonstrate evolutionary relationship of Oryza sativa CP-HSP with other monocots and dicots.