Efficient plant regeneration and in vitro rhizome induction of manyflower betony (Stachys floridana)


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Authors

  • JUAN LI
  • GUOYU ZHANG
  • ZHIHUI CHENG
  • WEIFU LIN

Keywords:

In vitro rhizome induction, Leaf explants, Manyflower betony, Micropropagation

Abstract

Manyflower betony (Stachys floridana Schuttl ex Benth) is a specific vegetable variety in China grown for rhizomes. An efficient protocol was developed for plantlet regeneration and in vitro rhizome induction to facilitate germplasm conservation, rapid propagation as well as breeding and production of this species. With this regeneration system, 79.8% of explants produced an average of 5.4 elongated shoots per explant, and 100% of shoots produced roots within 15 days. We also reported an efficient method for rapid in vitro rhizome induction from regeneration plantlets. This simple and efficient plant regeneration and in vitro microrhizome induction system could be useful for propagation of this vegetable and a promising approach to produce metabolites of interest, such as stachyose.

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Author Biographies

  • JUAN LI
    Assistant Professor, Rubber Research Institute, Chinese Academy of Tropical Agricultural
    Sciences, Danzhou, Hainan 571737, China;
  • GUOYU ZHANG
    Associate Professor, Beijing Vegetable Research Center,
    Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing 100097, China;
  • ZHIHUI CHENG
    Professor, College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shanxi 712100, China;
  • WEIFU LIN
    Professor , Rubber Research Institute, Chinese Academy
    of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China

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Issue

Vol. 83 No. 9 (2013)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Agricultural Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

LI, J., ZHANG, G., CHENG, Z., & LIN, W. (2013). Efficient plant regeneration and in vitro rhizome induction of manyflower betony (Stachys floridana). The Indian Journal of Agricultural Sciences, 83(9). https://epubs.icar.org.in/index.php/IJAgS/article/view/32845