Molecular marker-based linkage map of chickpea (Cicer arietinum) developed from desi × kabuli cross

Abstract

A molecular marker-based linkage map of chickpea (Cicer arietinum L.) was developed from a desi × kabuli cross of ‘BGD 112’ and ‘FLIP 90-166’ using Sequence-tagged Microsatellite (STMS) Markers. Both the parents representing the cultivated chickpea, the map loci marked indicates usable polymorphism for genetic as well as loci studies. A total of 250 STMS markers were used to study the parental polymorphism and 49 of which had showed polymorphism were used for genotyping F2 lines. Linkage analysis revealed 8 linkage groups mapped by 33 loci by these markers covering a distance of 471.1 cM of map distance with an average marker density of 14.2 cM at a LOD of 3.0. The molecular map using desi × kabuli cross throws insights into variability and diversity that can be utilized directly by the breeders unlike that generated using wide crosses as the loci that this map has marked, has a direct utility in marker-assisted breeding.