An efficient protocol of chemo-cum-thermotherapy for elimination of potato (Solanum tuberosum L.) viruses by meristem-tip culture
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Authors
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Jai Gopal
Central Potato Research Institute
Keywords:
Virus elimination, Potato viruses, Mericloning, Solanum tuberosumAbstract
With the objective to develop an efficient protocol for virus elimination from potato (Solanum tuberosum L.) clones, meristem-tip culture after chemotherapy, thermotherapy or chemo-cum-thermotherapy was attempted in a number of potato genotypes infected of PVX and PVS, the two most difficult viruses to eliminate. Chemotherapy was given by culturing the nodal cuttings from the infected plantlets for five weeks on MS medium containing 20 mg/l of ribavirin. For thermotherapy three weeks-old in vitro plantlets raised from nodal cuttings on MS medium were incubated at 37±1°C for two weeks. Chemo-cum-thermotherapy included chemotherapy for three weeks, followed by thermotherapy of the same plantlets on same medium for two weeks. Immuno-electron microscopy of the mericlones so raised clearly showed that chemo-cum-thermotherapy of the infected plants prior to mericloning was the best option for eliminating PVX and PVS as this treatment proved effective in freeing all accessions from these viruses. On the other hand chemotherapy alone was completely ineffective and thermotherapy was effective in a few genotypes. It is concluded that chemo-cum-thermotherapy protocol developed in the present study can be effectively used for cleaning a wide range of genotypes for all viruses by meristem-tip culture in potato, and probably also for other vegetatively propagated plant species.
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2010-08-26
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2011-03-31
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Gopal, J. (2011). An efficient protocol of chemo-cum-thermotherapy for elimination of potato (Solanum tuberosum L.) viruses by meristem-tip culture. The Indian Journal of Agricultural Sciences, 81(6). https://epubs.icar.org.in/index.php/IJAgS/article/view/422
