Studies on gamma ray induced mutants in gladiolus

ANJANA SISODIA; A K SINGH

doi:10.56093/ijas.v85i1.46003

Authors

ANJANA SISODIA Research Scholar, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005
A K SINGH Professor & Head, Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005

https://doi.org/10.56093/ijas.v85i1.46003

Keywords:

Chimeras, Flowering, Gamma irradiation, Gladiolus

Abstract

An experiment was conducted for 2 years (2011-12 to 2012-13) at Varanasi to find out the influence of various gamma rays doses, i e 1.5, 2.5, 3.5, 4.5 and 5.5 kr along with untreated (control) in different varieties of gladiolus, viz. Aldebaran, Jyotsna, Legend, Praha, Punjab Dawn, Pusa Kiran and Tiger Flame. Higher dose of gamma irradiation (5.5 kr) resulted in minimum sprouting. Maximum sprouting percentage was recorded with control followed by 1.5 kr, 2.5 kr, 3.5 kr, 4.5 kr and 5.5 kr. Gamma ray doses at 3.5 kr, 4.5 kr and 5.5 kr found were lethal in case of three varieties, i e Aldebaran, Legend and Praha and failed to produce any spike during 1^st year. Early spike emergence (74.37 days) was recorded with 1.5 kr dose of gamma irradiation. Late opening of 1^st floret (94.00 days) was observed with 2.5 kr of gamma dose. Flower opening was not observed at higher doses, i e 3.5 kr, 4.5 kr and 5.5 kr in cv. Pusa Kiran during 2^nd year. Maximum number of flowers/spike was recorded with control which was at par with lower dose of gamma irradiation (1.5 kr). Gamma dose at 2.5 kr exhibited maximum durability of 2^nd and 3^rd florets. Maximum length of spike at the last floret opening was noticed in control (74.48 cm) followed by 1.5 kr, 2.5 kr, 3.5 kr, 4.5 kr and 5.5 kr. During both years 2.5 kr resulted in maximum duration of flowering. Treatment 4.5 kr resulted in abnormal plant growth, bunched spike in some plants of cv. Pusa Kiran. A mutant having creamish white colour was found at 2.5 kr in one plant of cv. Jyotsna. A colour mutant was observed in cv. Tiger Flame. This colour mutant produced yellow coloured florets at 3.5 kr treatment. One mutant had been found in cv. Punjab Dawn at 2.5 kr treatment. Flowers were white in colour, whereas, flower colour of parent variety Punjab Dawn is pink. Colour of the flowers was white during VM1 and it was found stable in VM₂.

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References

Arnold N P, Barthakur N N and Tanguay M. 1998. Mutagenic effects of acute gamma irradiation on miniature roses: target theory approach. HortScience 33 (1): 127–9.

Bairagi R. 1969. Radiosensitive phase in plant germination. Proceedings 2nd Int. Conf., Peaceful Use of Atom in Geneva 27 pb, 299–304.

Banerji B K and Datta S K. 2002. Induction and analysis of gamma ray-induced flower head shape mutation in ‘Lalima’ chrysanthemum (Chrysanthemum morifolium). Indian Journal of Agricultural Sciences 72 (1): 6–10.

Banerji B K, Gupta M N and Datta S K. 1981. Effects of gamma irradiation on Gladiolus L. Cytomorphological studies on Gladiolus psittacinus var Hookeri cultivar Orange. North American Gladiolus Council Bulletin 148: 50–7.

Cantor M, Pop I and Korosfoy S. 2002. Studies concerning the effect of gamma radiation and magnetic field exposure on gladiolus. Journal of Central European Agriculture 3 (4): 277- 84.

Datta S K. 2012. Success story of induced mutagenesis for development of new ornamental varieties. Bioremediation, Biodiversity and Bioavailability, pp 15–26.

Datta S K. 1989. Improvement of ornamental plants through induced mutations. (In) Recent Advances in Genetics and Cytogenetic, pp 215–28. I A Khan, SA Farook (Eds).

Gunckel J E. 1957. The effects of ionizing radiation on plants. Morphological effects. Quantitative Review of Biology 32: 46– 56. DOI: https://doi.org/10.1086/401672

Kole P C and Meher S K. 2005. Effect of gamma rays of some quantitative and qualitative characters in Zinnia elegans N.J. Jacguin in M1 generation. Journal of Ornamental Horticulture 8 (4): 303–5.

Misra P, Banerji B K and Kumari A. 2009. Effect of gamma irradiation on chrysanthemum cultivar Pooja with particular reference to induction of somatic mutation in flower colour and form. Journal of Ornamental Horticulture 12 (3): 213–6.

Misra R L. 1982. A note of colour mutant in Gladiolus L. variety Ratna’s Butterfly. Haryana Journal of Horticulture Sciences 11 (3-4): 217–8.

Misra R L. 1986. Mutational studies in gladioli (Gladiolus L.) III. New forms as somatic mutants obtained by means of 60Co gamma rays. South Indian Horticulture 34 (5): 360–1.

Raghava S P S, Negi S S, Sharma T V R S and Balkrishna K A. 1988. Gamma ray induced mutants in gladiolus. Journal of Nuclear and Agriculture Biology 17 (1): 5–10.

Rather Z A and Jhon A Q. 2000. Effect of 60Co gamma rays on Dutch iris. Journal of Ornamental Horticulture 3 (2): 71–4.

Seilleur P. 1975. Effects of irradiation of gladiolus corms on the physiological behaviour of plants derived from them. Bulleten des Researches Agronomiques de Gembloux 10 (3): 291–306.

Singh A K and Kumar A. 2013. Studies of gamma irradiation on morphological characters in gladiolus. Asian Journal of Horticulture 8 (1): 299–303.

Srivastava P, Singh R P and Tripathi V K. 2007. Response of gamma radiation (60Co) on vegetative and floral characters of gladiolus. Journal of Ornamental Horticulture 10 (2): 135–6.

Venkatchalam P and Jayabalan N. 1992. Analysis of leaf protein in gamma rays induced mutants of zinnia. Crop Improvement 19: 97–9.