Molecular characterization of farmers' varieties of rice (Oryza sativa)


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Authors

  • VIJAYA KUMAR Assistant Professor, Department of Seed Science and Technology, College of Agriculture, UAS, Raichur, Karnatka 584 101
  • SUSHIL KUMAR Assistant Professor, Department of Agricultural Biotechnology, Anand Agricultural University, Anand, Gujarat 388 110
  • S K CHAKRABARTY Principal Scientist, Division of Seed Science and Technology, Indian Agricultural Research Institute, New Delhi 110 012
  • TRILOCHAN MOHAPATRA Director, Central Rice Research Institute, Cuttack, Orissa 753 006
  • MALAVIKA DADLANI Ex-Joint Director Research, Indian Agricultural Research Institute, New Delhi-110 012

https://doi.org/10.56093/ijas.v85i1.46063

Keywords:

Farmers' varieties, Rice, SSR markers, Seed characteristics

Abstract

A study was carried out for characterization of 60 farmers' varieties using grain characters and 40 SSR (Simple Sequence Repeat) markers. Only a set of 8 (20%) primers were polymorphic yielding 16 bands (alleles) among these 60 varieties. The size of amplicons ranged from 120 bp (RM238) to 240 bp (RM551). The number of alleles per microsatellite ranged from 1 (RM238, RM119 and RM120) to 3 (RM259, RM234 and RM551) with an average of 2 alleles per locus. Major allele frequency ranged from 0.51 to 0.97 averaging 0.74. Genetic diversity ranged from 0.045 to 0.588 with a mean of 0.34. Considering the entire genotypic array, the mean value for polymorphism information content (PIC) for all microsatellites was 0.27. Microsatellite RM122 with 2 alleles had the maximum PIC value (0.37) and the microsatellite RM205 with 2 alleles had the minimum (0.27) value. The UPGMA cluster analysis grouped varieties into three main clusters with 58% genetic similarity and homing 24, 23 and 13 varieties, respectively. Grain characteristic- based clustering was better than molecular markers as accessions were in expected cluster. However, few accessions showed scattering to other sub-clusters. The diversity analysis revealed the distinct nature of farmers' varieties in a large number of clusters indicating greater diversity, which could be exploited in breeding programmes to combine grain traits and early vigor.

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Submitted

2015-01-19

Published

2015-01-19

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Articles

How to Cite

KUMAR, V., KUMAR, S., CHAKRABARTY, S. K., MOHAPATRA, T., & DADLANI, M. (2015). Molecular characterization of farmers’ varieties of rice (Oryza sativa). The Indian Journal of Agricultural Sciences, 85(1), 118-124. https://doi.org/10.56093/ijas.v85i1.46063
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