A highly reliable DNA marker in dioecious plant Japanese hop (Humulus scandens)

Abstract

One hundred decamer primers of random amplified polymorphic DNA were tested on dioecious Humulus scandens plants to analysis genome difference. One of the 100 primers, S2142, produced one male-linked band (S2142₇₆₂), with the length of 762bp, rich in AT (54.7%). The marker was sequenced and specific primer was constructed to generate correspondingly size marker of sequence characterized amplified regions (SCAR) in male plants, SCAR₇₆₂, which is suitable for a precise, rapid identification of male plants in 30 male and female H. scandens plants, respectively. Southern hybridization, using S2142₇₆₂ as a probe, showed that S2142₇₆₂ exists in the male genomes, but not in the female genome. This result strongly suggests that S2142₇₆₂ is located on the chromosome region that is specific to the male.