Genetic diversity of Indian bitter gourd (Momordica charantia) by ISSR and morphological markers

PRATIBHA PANDEY; WAQUAR A ANSARI; SARVESH P KASHYAP; D R BHARDWAJ; SHAILESH K TIWARI; B SINGH

doi:10.56093/ijas.v89i12.96270

Authors

PRATIBHA PANDEY Senior Research Fellow, ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, 221 305, India
WAQUAR A ANSARI Research Associate, ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, 221 305, India
SARVESH P KASHYAP Senior Research Fellow, ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, 221 305, India
D R BHARDWAJ Principal Scientist, ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, 221 305, India
SHAILESH K TIWARI Scientist, ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, 221 305, India
B SINGH Director, ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, 221 305, India

https://doi.org/10.56093/ijas.v89i12.96270

Keywords:

Bitter gourd, Dendrogram, Genetic diversity, Morphological traits, ISSR

Abstract

Genetic variation among 29 bitter gourd genotypes was assessed using morphological and ISSR markers during 2013â€“14. High genetic variability was observed for yield per plant (397â€“1990 g), number of fruits per plant (9.18â€“43), individual fruit weight (25.47â€“125.67 g), plant height (110â€“503 cm), fruit length (6.39â€“25.97 cm), fruit diameter (2.53â€“6.1 cm), number of seeds (5â€“22.33) and number of branches per plant (4.67â€“16.44). The pair-wise Jaccardâ€™s similarity coefficient ranged between 0.22â€“1.00 based on morphological traits. The size of fragments varied from 200 bp (by ISSR marker UBC-808, 811, and 835) to 1550 bp (by ISSR marker UBC-807). Maximum polymorphism was shown by primers UBC-825 and lowest level of polymorphism was shown by UBC-807, UBC-809 followed by UBC-812, UBC-810. On an average 63.16% polymorphism was obtained per primer. All genotypes were grouped into five main clusters. Jaccardâ€™s similarity coefficient ranged from 0.73â€“1.00. These data revealed that large amount of genetic variability exist among the examined genotypes of bitter gourd.

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