Histological Study of Hypophysis Cerebri in Buffaloes (Bubalus bubalis)

Abstract

The hypophysis cerebri in adult buffalo was surrounded by a very thin capsule. The pars intermedia were distinguished from the pars nervosa by darkly staining cells lined with pseudostratified ciliated epithelium.The parenchyma of pars distalis composed of cells which included chromophil and chromophobe cells and arranged in clusters in the connective tissue framework with sinusoidal capillaries. By orange G-PAS staining method, acidophil cells were differentiated into type I and type II cells. Basophils cells were larger than acidophils. Chromophobe cells were rounded in shape and the cytoplasm was devoid of granules. The pars intermedia was well developed. Near the cleft, it was lined by pseudostratified ciliated columnar epithelium. Pars nervosa was composed of loose connective tissue framework with sinusoids, non myelinated nerve fibres and neuroglia like cells called pituicytes. The Herring bodies, aggregations of stained neurosecretory material of varying size, were found.