Identification of Sex of Domestic Ruminants by using PCR Technique


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Authors

  • Rupali Charjan Nagpur Veterinary College; Nagpur 440 006 (M.S.)

Keywords:

PCR, Ruminants, Sex identification, SRYgene

Abstract

The aim of this work was to identify the sex of domestic ruminants by using the PCR technique. Six  blood samples each from cattle, buffalo, sheep and goat of either sex were collected. The DNA extraction was conducted by using a DNAextraction kit. Extracted DNAwas subjected to PCR using the sex-determining region of the Y chromosome gene (SRY) as a biological marker. The PCR amplicon was loaded in 2% agarose gel and was run under a submersible electrophoresis machine. The band patterns were observed under U.V. Transilluminator. The banding pattern showed fragments at 122bp in all males, but no fragments were observed in
females in all the species under study.

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Author Biography

  • Rupali Charjan, Nagpur Veterinary College; Nagpur 440 006 (M.S.)

    Department of Anatomy and Histology

Additional Files

Submitted

2022-10-12

Published

2022-10-13

Issue

Vol. 34 No. 1 (2022): Indian Journal of Veterinary Anatomy

Section

Short Communications

License

As per the copyright policy of the Journal, authors would be required to delegate all the copyrights to the association.

How to Cite

Rupali Charjan. (2022). Identification of Sex of Domestic Ruminants by using PCR Technique. Indian Journal of Veterinary Anatomy, 34(1). https://epubs.icar.org.in/index.php/IJVA/article/view/129048