Comparative Study on the Use of Various Preservatives for Prolonged Preservation of Stained Sections of Brain
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Authors
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Priya Pachauri
Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India 281001
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Archana Pathak
Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India 281001
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Ankur Pandey
Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India 281001
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Rupam Sinha
Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India 281001
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Abhinov Verma
Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India 281001
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Ajay Prakash
Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India 281001
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M.M. Farooqui
Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India 281001
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Varsha Gupta
Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India 281001
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Shriprakash Singh
Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India 281001
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Anand Singh
Department of Veterinary Anatomy, College of Veterinary Science and Animal Husbandry U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India 281001
Keywords:
Brain, Epoxy resin, Glycerin, Preservation, SiliconAbstract
Formaldehyde is the most commonly used low-cost preservative, used for the preservation of anatomical specimens. However, the continuous use of formalin causes serious health problems and also deteriorates the natural colour of specimens. Therefore, efforts have been made to replace formalin with other preservatives which are not hazardous to health and maintain the quality of specimens for a prolonged time. The thin sections of the buffalo brain were stained with mulligan stain for the demonstration of gray and white substance. Each stained section of the brain was completely dehydrated in acetone and put into three preservatives viz. glycerin, epoxy resin and silicon. After complete processing, it was found that the section preserved in epoxy resin and silicon showed dark colour and were hard in consistency whereas, the glycerin- preserved specimen retained the maximum normal colour with normal consistency, and flexibility, and no shrinkage was noticed. Thus, it has been concluded that glycerin can be used as a good preservative instead of formalin for the prolonged preservation of brain.
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References
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