Histological, Histochemical, and Scanning Electron-Microscopical Studies on the Vestibular Region of Nasal Cavity in Dogs
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Authors
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Shikha Saini
College of Veterinary Sciences Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, India
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Pawan Kumar
College of Veterinary Sciences Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, India
Keywords:
Vestibule, Alar fold, Straight fold, Dogs, Scanning electron-microscopyAbstract
The present study was conducted to determine the histological, histochemical, and scanning electron-microscopical details of the vestibular region of the nasal cavity in adult dogs. The mucosa of the vestibular region (straight and the alar folds, and rostral portions of the dorsal and ventral nasal turbinates) was lined by a non-keratinized stratified squamous epithelium. The superficial part of the propria submucosa contained different connective tissue cells, collagen, elastic, and reticular fibres in various concentrations, isolated sero-mucous glands, fine blood capillaries, small blood vessels, and venous caverns-like structures. The deeper portion featured large clusters of sero-mucous glandular acini along with their ducts, nerve bundles, and blood vessels. Hyaline cartilage in the deeper part formed a supportive framework. The stratified squamous lining, gradually transitioned caudally into stratified cuboidal (transitional zone) to pseudostratified columnar type with occasional goblet cells. The glandular units reacted differently to various histochemical stains revealing different moieties of carbohydrates but these glands were lacking proteins in their secretions. SEM presented small, irregular, scale-like flat squamous cells which were continuous with each other, displaying microplicae in various arrangements at higher magnification. In the region of transition, a few ciliated cells started appearing along with some raised cells bearing microvilli of different shapes and sizes and a few goblet cells.
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Abdel-Salam, L.R., Hussein, S.A., Gad, M.H., Khattal, A.A.A. and Amer, A.H. 2014. Histological, histochemical and scanning electron microscopical study of nasal cavity epithelium of the dromedary camel (Camelus dromedarius). Journal of Basic Medical and Allied Sciences 2 : 1-24.
Adams, D.R. 1986. Transitional epithelial zone of the bovine nasal mucosa. The American Journal of Anatomy 176 : 159-170.
Adams, D.R. and Hotchkiss, D.K. 1983. The canine nasal mucosa. Anatomia Histologia Embryologia 12 : 109-125.
Al-Kafagy, S.M., and Reshag, A.F. 2020. Histological features of the nasal cavity in indigenous dogs. Plant Archives 20 : 7979-7984.
Badawi, H. and El-Bab, M.R.F. 1974. Anatomical and histological studies on the nasal cavity of the camel (Camelus dromedarius). Assiut Veterinary Medical Journal 1 : 1-14.
Blatt, C.M., Taylor, C.R., and Habal, M.B. 1972. Thermal panting in dogs: the lateral nasal gland, a source of water for evaporative cooling. Science 177 : 804-805.
Bradshaw, J.W.S. 1992. The Waltham Book of Dog and Cat Behavior. pp. 31-52. Thorne, C. (Edt.), Pergamon Press, Oxford.
Chamanza, R., and Wright, J.A. 2015. A review of the comparative anatomy, histology, physiology and pathology of the nasal cavity of rats, mice, dogs and non-human primates. Relevance to inhalation toxicology and human health risk assessment. Journal of Comparative Pathology 153 : 287-314.
Crossmon, G. 1937. A modification of Mallory's connective tissue stain with a discussion of principles involved. The Anatomical Record 69 : 33-38.
Dellmann, H.D. 1993. Textbook of Veterinary Histology. 4th edn., Lea and Febiger, Philadelphia, USA.
El-Bakary, R., Enany, E.S.E., Karkoura, A.A., Abumandour, M., and Gafaar, S.F. 2019. Histological characterizations of the nasal conchae of the dog (Canis lupus). Alexandria Journal of Veterinary Sciences 60 : 42-47.
Ganganaik, S., Jain, R.K. and Kumar, P. 2009. Histological studies on the nasal cavity of sheep (Ovis aries). Haryana Veterinarian 48
: 68-71.
Ganganaik, S., Jain, R.K., Kumar, P. and Gupta A.N. 2007. Scanning electron microscopic studies on the nasal cavity of sheep (Ovis aries). Indian Journal of Animal Sciences 77 : 878-879.
Gewaily, M., Hadad, S., and Shoghy, K. 2019. Gross, histological and scanning electron morphological studies on the nasal turbinates of one humped camel (Camelus dromedarius). Bioscience Research 16 : 107-120.
Girgiri, I.A., Malah, M.K., Jiji, M.H. and Gazali,
Y.A. 2022. Gross and histological studies of the nasal turbinates in Yankasa sheep (Ovis aries) in Maiduguri, Nigeria. Sahel Journal of Veterinary Sciences 19 : 13-20.
Hafez, E.S.E. 1962. The Behavior of Domestic Animals. 1st edn., Bailliere Tindall and Cox, London.
Kahwa, C.K.B. and Balemba, O.B. 1998. The non-olfactory nasal epithelium in the adult goat: a scanning electron microscopic study. Small Ruminant Research 29 : 277-282.
Kahwa, C.K.B. and Purton, M. 1996. Histological and histochemical study of epithelial lining of the respiratory tract in adult goats. Small Ruminant Research 20 : 181-186.
Kalita, A. 2014. Histomorphological study of the respiratory system of Mizo local pig (Zo Vawk). Asian Journal of Biomedical and Pharmaceutical Sciences 4 : 50-54.
Khamas, W.A.H. and Ghoshal, N.G. 1982. Histomorphologic studies of the nasal cavity of sheep (Ovis aries) and its significance in temperature regulation of the brain. Acta Anatomica 113 : 340-351.
Kumar, P., Timoney, J.F., Southgate, H.H.P. and Sheoran, A.S. 2000. Light and scanning electron microscopic studies of the nasal turbinates of the horse. Anatomia Histologia Embryologia 29 : 103-109.
Kumar, Pawan, Gupta, A.N., and Nagpal, S.K. 2008. Scanning electron microscopy of the nasal cavity of the buffalo calves (Bubalus bubalis). Indian Journal of Animal Sciences 78 : 1094-1097.
Kumar, Pawan, Kumar, S. and Singh, Y. 1992. Histological studies on the nasal turbinates of goat. Indian Journal of Animal Sciences 4
: 75-79.
Kumar, Pawan, Kumar, S. and Singh, Y. 1994. Histology of nasal turbinates in dog. Indian Journal of Animal Sciences 64 : 1050-1053.
Kumar, Pawan, Kumar, S. and Singh, Y. 1999. Scanning electron-microscopic studies on the nasal cavity of goat. Indian Journal of Animal Sciences 69 : 887-890.
Larochelle, R., and Martineau-Doize, B. 1990. Distribution of epithelia in the nasal cavity of piglets. Acta Anatomica 139 : 214-219.
Luna, L.G. 1968. Manual of Histologic Staining Methods of Armed Forces Institute of Pathology. 3rd edn., McGraw Hill Book Co., New York.
Magilton, J.H., and Swift, C.S. 1968. Description of two physiological heat exchange systems for the control of brain temperature. IEEE Conference Record: Annual Rocky Mountain Bioengineering Symposium 5 : 24-
27.
Manjunatha, K., Jamuna, K.V., Parsad, R.V., Naryanaswamy, H.D., Sanganal, J.S., and Girish, M.H. 2018. Histological study of nasal cavity in pigs. International Journal of Chemical Studies 6 : 1658-1660.
Mustafa, A.R., and Reshag, A.F. 2018. Histological study on the nasal cavity of black Iraqi goat (Capra hircus). The Iraqi Journal of Veterinary Medicine 42 : 105-111.
Parkash, T., and Kumar, Pawan. 2019. Histological and histochemical studies on the nasal cavity of the young pigs (Sus scrofa). Indian Journal of Veterinary Anatomy 31 : 40-43.
Pathak, V., and Rajput, R. 2018. Histological and histochemical studies on the nasal cavity of Gaddi sheep. Indian Journal of Small Ruminants 24 : 281-287.
Pearse, A.G.E. 1968. Histochemistry: Theoretical and Applied. 3rd edn., Vol. 2. Churchill Livingstone, London.
Qin, T., Yin, Y., Huang, L., Yu, Q. and Yang, Q. 2015. H9N2 influenza whole inactivated virus combined with polyethyleneimine strongly enhances mucosal and systemic immunity after intranasal immunization in mice. Clinical and Vaccine Immunology 22 : 421-429.
Scott, J.H. 1954. Heat regulating function of the nasal mucous membrane. The Journal of Laryngology & Otology 68 : 308-317.
Suman, Singh, G. and Nagpal, S.K. 1998. Histological studies on the nasal cavity of Indian camel (Camelus dromedarius). Journal of Camel Practice and Research 5 : 99-104.
Tomosada, Y., Chiba, E., Zelaya, H., Takahashi, T., Tsukida, K. and Kitazawa, H. 2013. Nasally administered Lactobacillus rhamnosus strains differentially modulate respiratory antiviral immune responses and induce protection against respiratory syncytial virus infection. BMC immunology 14 : 1-16.
Walker, J.E.C., Wells Jr, R.E. and Merrill, E.W. 1961. Heat and water exchange in the respiratory tract. The American Journal of Medicine 30 : 259-267.
Yang, J., Dai, L., Yu, Q. and Yang, Q. 2017. Histological and anatomical structure of the nasal cavity of Bama minipigs. PLoS One 12
: 1-14.
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