Histoenzymic Localization of Oxidoreductases and Phosphatases in the Tonsils of Naso-pharyngeal region of Buffaloes
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Authors
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Pawan Kumar
Department of Veterinary Anatomy, College of Veterinary Sciences Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 004 (Haryana)
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Amandeep Singh
Department of Veterinary Anatomy, College of Veterinary Sciences Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 004 (Haryana)
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Tej Parkash
Department of Veterinary Anatomy, College of Veterinary Sciences Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 004 (Haryana)
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Parveen Kumar Gahlot
Department of Veterinary Anatomy, College of Veterinary Sciences Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 004 (Haryana)
Keywords:
Buffalo, Oxidoreductases, Phosphatases, TonsilsAbstract
The present study was aimed to explore the localization of oxidoreductases and phosphatases enzymes in the nasopharyngeal, tubal and paraepiglottic tonsils of six adult healthy buffaloes. The succinic dehydrogenase, lactic dehydrogenase, glucose-6-phosphate dehydrogenase and glutamic dehydrogenase showed a strong positive activity in the pseudostratified columnar ciliated epithelium and stratum basale layer of the stratified squamous
epithelium of the tonsils. The diffuse lymphoid tissue and the striated muscles showed moderate to strong
positive reactions. The mucous secretory acini did not show any affinity for these enzymes, however, a uniform
moderate to strong positive reaction was observed towards their basement membranes. The acid and glucose-
6-phosphatases showed a moderate activity in the respiratory epithelium and the deepest layer of cells next to
the basement membrane of the stratified squamous epithelium. The associated submucosal lymphoid tissue
and the glandular tissue of the tonsils showed positive reactions like those of the oxidoreductases group of enzymes. A comparison of the pattern of localization of these enzymes in healthy and infected animals may
play a significant role in disease diagnosis, if any.
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