Histochemical Dynamics of Ovarian Tissue across Developmental Stages in sheep
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Authors
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Mir Shabbir Ahmad
Department of Veterinary Anatomy and Histology, SKUAST-K, Srinagar, 190001, India,
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Massarat Khan
Department of Veterinary Anatomy and Histology, SKUAST-K, Srinagar, 190001, India,
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Masuood Ahmad John
Department of Veterinary Anatomy and Histology, SKUAST-K, Srinagar, 190001, India,
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A.R.Choudhury
Department of Veterinary Anatomy and Histology, SKUAST-K, Srinagar, 190001, India,
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S.A.Shah
Department of Veterinary Anatomy and Histology, SKUAST-K, Srinagar, 190001, India,
Keywords:
Histochemistry, Ovarian tissue, Follicular development, SheepAbstract
Seasonal variation had no appreciable effect on histochemical reactions; however, clear age-related differences were observed. Neutral mucopolysaccharides (PAS-positive substances) showed generally weak reactions in prepubertal animals, increasing to mild–moderate intensity in pubertal and adult groups, particularly in follicular components, zona pellucida, corpus luteum, and stroma. Glycogen content was uniformly low or absent across most ovarian structures, irrespective of age. Acid mucopolysaccharides exhibited mild to moderate reactivity in stromal tissue, follicular layers, basement membrane, zona pellucida, and follicular fluid, with comparatively higher activity in developing and tertiary follicles. Basic proteins were consistently present in most ovarian structures with moderate to strong reactions in granulosa cells, basement membranes and zona pellucida of growing follicles. Lipids and phospholipids showed mild to moderate distribution in follicular cells, luteal tissue, and stroma with relatively higher lipid content in prepubertal ovaries and a decreasing trend with advancing age.
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References
Bancroft, J.D. and Stevens, A. 1996.Theory and practice Practice of Histological Techniques.4th Ed. Churchill and
Livingstone. London.
Bansal, N., Uppal, V. and Kumar, A. 2008. Histomorphometrical and histochemical studies on the ovaries of domestic cat (Felis catus). Indian Journal of Animal Sciences, 78(2): 175-177.
Bhardwaj, R.L. and Roy, K.S. 1997. Age related histochemical studies on the ovary of Indian buffalo (Bubalus bubalis). Indian Journal of Veterinary Anatomy, 9(1/2): 1 -14.
Bhattacharya, M. and Saigal, R.P. 1990. Histoenzyme studies on the goat ovarian steroid secreting cells. Indian Journal of Animal Sciences, 60 : 398-400.
Chayen, J., Butcher, R.G, Bitensky, L. and Poulter, L.W. 1969. A Guide to Practical Histochemistry. pp 83-174.
Chemineau, P., Malpaux, B., Delgadillo, J.A., Gurein, Y., Ravault, J.P., Thimonier, J. and Pelletier, J. 2004. Control of sheep and goat reproduction: use of light and melatonin. Animal Reproduction Science, 30: 157-184.
Dwivedi, J.N. and Singh, C.M. 1971. Histochemical Observations on the Ovary of Indian Buffalo. Indian Journal of
Animal Health, 10: 205-211. Emara, S.A., Hassanin, A.M.A. and Elnagar, H.Y. 2019. Histological and Histochemical
Changes of The Ovary of Goat During Autumn and Summer Seasons. Journal of Current Veterinary Research, 2: 47-54.
Fields, M.J. and Fields,P.A.1996.Morphological characteristics of the bovine corpus luteum during the estrous cycle and pregnancy. Thereiogenology, 45: 1295-1326.
Guigon, C.J. and Magre, S. 2006. Contribution of germ cells to the differentiation and maturation of the ovary: insight from models of germ cell depletion. Biology of Reproduction, 75: 450-58.
Guraya, S.S. 1997. Ovarian Biology in Buffaloes and Cattle. Indian Council of Agricultural Research, New Delhi.
Islam, M.M. and Das, P. 2019. Histomorphological Study on Gravid and Non-Gravid Ovaries of Garole Sheep. Indian Journal of Veterinary Anatomy, 31(1): 49-51.
Luna, L.G. 1968. Manual of histological staining methods of the Armed forces institute of Pathology, 3rd Edn. The Blakistan Division, Mo-Graw Hill Book Company, New York
Prasad, M., Venugopal, S.P and Babu, R.S. 2017. A gross and histological study of human foetal ovary at different weeks of gestation. International Journal of Anatomy and Research, 5(2): 4006-11
Rajput, R. and Sharma, D.N. 1996. Histological and histochemical studies on the ovary of cyclic Gaddi sheep. Indian Veterinary Journal, 73(7): 757 -761.
Sahu, S.K., Das, R.K., Sathapathy, S., Mishra, U.K and Dash, S.K. 2017. Gross, histological and histochemical studies on the ovary and oviduct of Kendrapada sheep (Ovis aries) at different age groups. Journal of Entomology and Zoology Studies, 5: 2319- 2324.
Sasan, J.S., Uppal, V. and Bansal, N. 2013.Histochemical localization of mucopolysaccharides, proteins and lipids
in ovarian tissue: A seasonal study. Indian Journal of Animal Sciences, 83(2): 150–153.
Sastry, N.S.R., Thomas, C.K. and Singh, R.A. 1982. Farm Animal Management and Poultry Production, Fifth Edition. Vikas Publishing House, New Delhi pp. 15-28.
Shehan, N.A., Adel Kareem, D. and Ali, S.A. 2017. Morphological and Histological of Ovary in Domestic Iraqi Sheep (Ovis aries). International Journal of Science and Research, 6(2): 284-291.
Singh, O. 1994. Histomorphological and histochemical studies on corpus luteum of buffalo (Bubalus bubalis). M.V.Sc. Thesis, Punjab Agricultural University, Ludhiana, India.
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