Histoenzymic Localization in Pancreas of Buffalo During Prenatal Life

Abstract

The present study was conducted on pancreas of six buffalo foetii divided into three groups based on their crown vertebral rump length (CVRL) viz; group I (0-20 cm), group II (20-40 cm) and group III (above 40 cm). The cryostat sections of 10 μ of fresh unfixed tissues were incubated in different substrates for histoenzymic localization of phosphatases, oxidoreductases and esterases. The study revealed a variable activity of Alkaline phosphatase, glucose-6-phosphatase, succinic dehydrogenase, lactic dehydrogenase, glutamate dehydrogenase, nicotinamide adenine dinucleotide hydrogenase diaphorase, glucose 6-phosphatase dehydogenase and non-specific esterases in different parts of pancreas during different stages of gestation. The enzymic activity has been correlated with their physiological function.