Transmission Electron Microscopic and Sperm Function Study of Spermatozoa in Murrah Buffalo Bull (Bubalus bubalis)

Abstract

The mature spermatozoon consisted of head which was covered by the acrosome. Neck showed a depression (fossa) which was continuous to middle piece consisted of a flagellar core and fibers. Middle piece was followed by principal or main piece and terminated in an end piece. In sperm function test, hypo-osmotic swelling sperm percentage and acrosome intact percentage of Murrah buffalo bull semen were 89.17±1.87 % and 92.33± 0.99 % respectively for hundred spermatozoa. It can be concluded that sperm morphometric characterization assisted by electron microscope and sperm function test in neat semen of Murrah buffalo bull may provide fast and reproducible measurements to indicate good quality semen.