Histochemical Variations in Buffalo Testis During Different Seasons

Abstract

The study was conducted on the testis of twenty four apparently healthy buffaloes collected from the slaughter house. Depending upon the season of the year, the animals were divided into four groups with six animals in each group viz; Group I: Spring season (from March to May), Group II: Summer seasons (from June to August), Group III: Autumn season (from September to November) and Group IV: Winter (from December to February). The study revealed that the tunica fibrosa and vasculosa layers showed weak PAS and alcian blue reactions in spring and summer season which became moderate in autumn and winter season. The basement membrane of seminiferous tubules showed the presence of both acid and neutral mucopoysachharides in
summer which became moderate to strong during spring, autumn and winter. Leydig cells revealed weak PAS positive activity during summer which became weak to moderate in autumn, and moderate in spring and winter. The basal cells of seminiferous tubules had more intensity of reaction for basic proteins which decreased towards the luminal border in all the seasons, whereas reverse pattern was observed for Sudanophilic lipids.