Culture Methods for Isolation of Canine Hair Follicle Stem Cells (CHFSC)
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Authors
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N. Hemavathi
Madras Veterinary College
Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007 (T.N.)
Keywords:
Key words, Stem cells, Canine, Hair follicle, Micro-dissection, CFU, YieldAbstract
Adult stem cell models are important tools interacting in tissues, replacing animal experimental studies for understanding the basis of physiology and therapeutics. In this study, we aimed to isolate and culture stem
cells from canine hair follicular tissue in vitro by three methods viz, explants cuture, microdissection and filtrate culture methods. Dispase II enzymatical digestion gave higher cell yield (3.08 ± 0.47×106) than trypsin/EDTA
enzymatical digestion of canine skin samples. Filtrate culture method gave high cell yield among three different culture methods. In microdissection method, colony forming unit (CFU) and colony size of cells from isthmus fragment were 14.71 ± 1.57 in numbers and 11.21 ± 2.45 mm- 2 respectively which was significantly higher than other fragments of hair follicle (P<0.01). We concluded that the dispase II enzyme digestion and filtrate culture method was the best method to isolate cHFSCs.