PROTECTIVE EFFECT OF AQUEOUS EXTRACT OF Phyllanthus amarus ON THE HAEMATOBIOCHEMICAL PARAMETERS OF GLYPHOSATE INDUCED TOXICITY IN WISTAR RATS
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Authors
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R. Winston Joel
Postgraduate scholar, Department of Veterinary Pathology, Madras Veterinary College, TANUVAS
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N. Pazhanivel
Professor, Department of Veterinary Pathology, Madras Veterinary College, TANUVAS, Chennai
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S. Vairamuthu
Professor and Head, Centralised Clinical Laboratory, MVC, TANUVAS, Chennai
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S.P. Preetha
Assistant Professor, Department of Veterinary Pharmacology and Toxicology, MVC, TANUVAS, Chennai
Keywords:
Glyphosate toxicity, Haematobiochemical parameters, Phyllanthus amarus, Wistar ratsAbstract
Glyphosate is an organophosphorus herbicide widely used in agricultural practices. The present study was conducted to find out the protective effect of P. amarus aqueous extract against hematobiochemical alterations caused by glyphosate toxicity in Wistar rats. About 60 Wistar rats were randomly (n=12) divided into five groups: Group 1 (Control group), Group 2 (Glyphosate (GLP)-500 mg/kg),Group 3 (Phyllanthus amarus (PA)-100 mg/kg aqueous extract), Group4 (Glyphosate 500 mg/kg +Phyllanthus amarus 100 mg/kg aqueous extract-GLP +PA prophylactic) and Group 5 (Glyphosate 500 mg/kg (Upto 9 days) + Phyllanthus amarus 100 mg/kg aqueous extract-GLP+PA therapeutic (10th to 28th day)}. Haematological parameters, liver specific enzymes (ALT, AST and ALP), kidney function tests (BUN and creatinine), serum electrolyte and minerals were studied in glyphosate induced toxicity. Phyllanthus amarus extract (Prophylactic and Therapeutic) showed highly significant reduction ((P<0.01) in the Haemoglobin, PCV and TEC compared with glyphosate group. Numerical reduction in the values of ALT, AST and ALP in the treatment groups compared with glyphosate group. Significant (P<0.05) reduction in the values of BUN and creatinine observed in treatment groups compared with glyphosate group. Significant (P<0.05) reduction in the value of sodium observed in treatment groups compared with glyphosate group.The results indicated that P.amarus had partially protective effect on haematobiochemical changes caused by glyphosate toxicity in Wistar rats.
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References
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