VISUAL DETECTION OF RECOMBINASE POLYMERASE AMPLIFIED DNA FOR RAPID DIAGNOSIS OF LEPTOSPIROSIS IN CANINE
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Authors
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Senthilkumar K
Assistant Professor, ZRL, DCAHS, Tamil Nadu Veterinary and Animal Sciences University
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Nirmala K
Project Associate, ZRL, DCAHS, Tamil Nadu Veterinary and Animal Sciences University
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Tirumurugaan K.G
Professor, ZRL, DCAHS,Tamil Nadu Veterinary and Animal Sciences University
Keywords:
Canine, leptospirosis, recombinase polymerase amplification assayAbstract
Rapid, sensitive and accurate diagnosis of Leptospirosis in canines is very much helpful in areas of increased prevalence. This study aimed to visualise Recombinase Polymerase amplified DNA of pathogenic Leptospira for rapid diagnosis of Leptospirosis in canine. The Recombinase Polymerase Amplification (RPA) assay for amplifying the lipL32 gene of pathogenic Leptospira was optimised. The visual detection of the amplified DNA by incorporating an optimised concentration of SYBR Green I dye in an indigenously built low-cost detection device was also optimised. Light green fluorescence was observed in the positive samples, while the negative samples appeared orange. The visual detection format of the RPA can be integrated as a point of a diagnostic test in any veterinary clinic or in remote areas where resource settings are limited.
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References
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