Protein Profile of Granulosa Cells in Cyclic and Acyclic Buffaloes
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Authors
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S. Satheshkumar
Professor and Head, Department of Veterinary Gynaecology and Obstetrics, Veterinary College and Research Institute, Tamilnadu Veterinary and Animal Sciences University (TANUVAS), Tirunelveli – 627 358
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B. Revathipriya
M.Phil. Scholar, Department of Animal Biotechnology, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 051, Tamilnadu
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S. Sakthivel
Senior Research Fellow, Department of Animal Biotechnology, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 051, Tamilnadu
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K. Brindha
Associate Professor, Department of Animal Biotechnology, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 051, Tamilnadu
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M. Parthiban
Professor and Head, Department of Animal Biotechnology, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 051, Tamilnadu
Keywords:
Buffaloes, Anoestrus, Follicular cells, Protein profile analysisAbstract
The objective of the study is to analyze the alterations in the protein profile of follicular cells in normal cyclic and acyclic anoestrus water buffaloes. Ovaries from sexually mature buffaloes were collected from abattoir and categorized into two groups viz., i. Cyclic and ii. Acyclic. In both the categories of ovaries, the follicular fluid (FF) was aspirated from all large follicles (> 9mm diameter) and small follicles (< 9 mm diameter) separately. FF was centrifuged and four categories of pelleted granulosa cells (CSG and ASG: Granulosa cells of small follicles in cyclic and acyclic groups respectively; CLG and ALG: Granulosa cells of large follicles in cyclic and acyclic groups respectively) were subjected for SDS-PAGE analysis. A total of 30 and 18 bands (from 7.1 to 209.0 kDa) were observed in CSG and CLG categories, while 16 bands in each of the ASG and ALG were recorded. It was observed that 40 per cent of bands present in the CSG group were not observed in CLG group. On the contrary, almost all the proteins found in ASG group were retained in the ALG group. It can be inferred that a group of small molecular weight granulosa cell proteins, correlating to IGF / IGFBP system, play a key role in providing a favorable proteo-genomic environment in the early stages of follicular development. In acyclic animals, retaining of such proteins in the large follicles indicated that the non-availability of metabolic factors needed for final maturation of follicles.
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References
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