Multiple Infections in Pacific White Shrimp (Penaeus vannamei) with Black Gill Disease
76 / 14
Authors
-
A.Uma
Professor and Head, State Referral Laboratory for Aquatic Animal Health, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, MMC,Chennai-600051
-
G.Rebecca
Senior Research Fellow, State Referral Laboratory for Aquatic Animal Health, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, MMC,Chennai-600051.
-
P.Karthik
Senior Research Fellow, State Referral Laboratory for Aquatic Animal Health, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, MMC,Chennai-600051.
-
S.Gangatharan
Senior Research Fellow, State Referral Laboratory for Aquatic Animal Health, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, MMC,Chennai-600051.
-
S.Ganesh Babu
Senior Research Fellow, State Referral Laboratory for Aquatic Animal Health, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, MMC,Chennai-600051.
Keywords:
Penaeus vannamei, Black gill diseases, WSSV, Aspergillus sp.Abstract
Penaeus vannamei (Pacific white shrimp) is the most extensively farmed among the crustaceans globally. Disease is the major impeding factor in the commercial culture of P vannamei undertaken in freshwater, brackish and marine aquaculture systems. In this study, P. vannamei samples were collected from a commercial shrimp farm at Tiruvallur district, Tamilnadu that has reported black gill disease. The clinical symptoms observed in the shrimp were melanized black gills, reduced feeding, surfacing and mortality in the early morning. Samples of gills were collected aspectically and diagnosed for bacterial Acute Hepatopancreatic Necrosis Disease (AHPND); viral diseases viz., Gill Associated Virus (GAV), White Spot Syndrome Virus (WSSV), Yellow Head Virus (YHV), Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and fungal infections by conventional diagnostic methods and PCR following confirmation by nucleotide sequencing. The results of the study showed that P. vannamei infected with black gill disease had multiple infections with fungus, Aspergillus niger and virus WSSV.
Downloads
References
Anshary, H and Baxa, D.V. (2017). Multiple viral pathogens occurrence in tiger shrimp (Penaeus monodon) broodstock from Sulawesi coastal waters. Aquaculture, Aquarium, Conservation & Legislation, 10(4):936-950.
Barnett, H.L and Hunter, B.B. (1999). Illustrated genera of imperfect fungi (p. 218). St. Paul: APS.
Belton, B and Thilsted S. H. (2014).Fisheries in transition: Food and nutrition security implications for the global South. Global Food Securitypp.59-66.
Chatterjee, S and Haldar, S. (2012). Vibrio related diseases in aquaculture and development of rapid and accurate identification methods. Journal of Marine Science Research and Development , 1.
Colorni A. (1989) Fusariosis in the shrimp Penaeus semisulcatus cultured in Israel. Mycopathologia, 108:145-147.
Corbel, V., Zuprizal, Z., Shi, C., Arcier, J.M and Bonami, J.R. (2001).
Experimental infection of European crustaceans with white spot syndrome virus (WS SV). Journal of fish diseases, 24(7):377-382.
Cowley, J.A., Dimmock, C.M., Spann, K.M and Walker, P.J. (2000). Detection of Australian gill-associated virus (GAV) and lymphoid organ virus (LOV) of Penaeus monodon by RT¬nested PCR. Diseases of aquatic organisms, 39:159-167.
Cowley, J.A., Cadogan, L.C., Wongteerasupaya, C., Hodgson, R.A., Boonsaeng, V and Walker, P.J. (2004). Multiplex RT-nested PCR differentiation of gill-associated virus (Australia) from yellow head virus (Thailand) of Penaeus monodon. Journal of virological methods, 117(1):49-59.
Dewangan, N.K., Gopalakrishnan, A., Kannan, D., Shettu, N and Singh, R.R. (2015). Black gill disease of Pacific white leg shrimp (Litopenaeus vannamei) by Aspergillus flavus. Journal of Coastal Life Medicine, 3(10):761-765.
Durand, S.V., Tang, K.F.J and Lightner, D.V. (2000). Frozen commodity shrimp: potentialavenue for introduction of white spot syndrome virus and yellow head virus. Journal of Aquatic Animal Health, 12(2) : 128-135.
Fernand, M.G., Roullier, C., Guitton, Y., Lalande, J., Lacoste, S., Dupont, J., Ruiz, N., Pouchus, Y.F., Raheriniaina, C. and Ranaivoson, E. (2017). Fungi isolated from Madagascar shrimps- investigation of the Aspergillus niger metabolism by combined LC-MS and NMR metabolomics studies. Aquaculture, 479:750-758.
Gardes, M and Bruns, T.D. (1993). ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Molecular ecology, 2(2):113-118.
Hatai K and Egusa S. (1978). Studies on the pathogenic fungus associated with black gill disease of kuruma prawn Penaeus japonicus II: some of the note on the BG-Fusarium. Fish Pathology 12:225-231. (in Japanese, with English abstract).
Ishikawa, Y. (1968). A fungus caused black gill condition in cultured kuruma prawn. Fish. Pathology, 3:34-49.
Khoa, L.V., Hatai, K and Aoki, T. (2004). Fusarium incarnatum isolated from black tiger shrimp, Penaeus monodon Fabricius, with black gill disease cultured in Vietnam. Journal of fish diseases, 27(9) : 507-515.
Kusumaningrum, H.P and Zainuri, M. (2015). Detection of bacteria and fungi associated with Penaeus monodon postlarvae mortality. Procedia Environmental Sciences, 23:329-337.
Lavilla-Pitogo, C.R., Lio-Po, G.D., Cruz¬Lacierda, E.R., Alapide-Tendencia, E.V and De la Pena, L.D. (2000). Diseases of penaeid shrimps in the Philippines.2nd ed. Iloilo: Aquaculture Department Southeast Asian Fisheries Development Center.
Lightner, D.V. (1996). A handbook of shrimp pathology and diagnostic procedures for diseases of cultured penaeid shrimp. World Aquaculture Society, Baton Rouge, pp.256.
Lightner, D.V., Redman, R.M., Poulos, B.T., Nunan, L.M., Mari, J.L and Hasson, K.W. (1997). Risk of spread of penaeid shrimp viruses in the Americas by the international movement of live and frozen shrimp. Revue scientifiqueet technique-Office international des epizooties, 16:146-160.
Lightner, D.V. (2011). Virus diseases of farmed shrimp in the Western Hemisphere (the Americas): a review. Journal of invertebrate pathology, 106(1):110-130.
Manivannan, S., Otta, S.K., Karunasagar, I and Karunasagar, I., (2002). Multiple viral infection in Penaeus monodon shrimp postlarvae in an Indian hatchery. Diseases of aquatic organisms, 48(3): 233-236.
MPEDA. (2017). Ministry of commerce and industry. 2017 Annual report. https:// mpeda.gov.in /MPEDA/admin/ app/webroot/files/annual report.
Ramaiah N. (2006). A review of fungal disease of algae, marine fishes, shrimp and corals. Indian journal of marine sciences, 35(4):380-387.
Rhoobunjongde, W., Hatai, K., Wada, S and Kubota, S. S. (1991). Fusarium moniliforme (Sheldon) isolated from gills of kuruma prawn Penaeus japonicus (Bate) with black gill disease. Nippon Suisan Gakkaishi, 57(4):629-35.
Shigueno, K.. (1975). Shrimp culture in Japan. Assoc. for International Technical Promotion, Tokyo, Japan: 153p.
Soegianto, A., Charmantier-Daures, M., Trilles, J.P. and Charmantier, G. (1999). Impact of copper on the structure of gills and epipodites of the shrimp Penaeus japonicus Journal of Crustacean Biology, 19:209-223.
Spann, K.M., Cowley, J.A., Walker, P.J and Lester, R.J.G. (1997). A yellow-head¬like virus from Penaeus monodon cultured in Australia. Diseases of aquatic organisms, 31(3):169-179.
Takahashi, Y., Itami, T., Maeda, M., Suzuki, N., Kasornchandra, J., Supamattaya, K., Khongpradit, R., Boonyaratpalin, S., Kondo, M., Kawai, K., Kusuda, R., Hirono, I and Aoki, T. (1996). Polymerase chain reaction (PCR) amplification of bacilliform virus (RV¬PJ) DNA in Penaeus japonicus Bate and systemic ectodermal and mesodermal baculovirus (SEMBV) DNA in Penaeus monodon Fabricius. Journal of Fish Diseases. 19:399-403.
Tang, K.F., Navarro, S.A and Lightner, D.V. (2007). PCR assay for discriminating between infectious hypodermal and hematopoietic necrosis virus (IHHNV) and virus-related sequences in the genome of Penaeus monodon. Diseases of aquatic organisms, 74(2):165-170.
White, T.J., Bruns, T., Lee, S.J.W.T and Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications, 18(1):315-322.
Zhan, W.B., Wang, Y.H., Fryer, J.L., Yu, K.K., Fukuda, H and Meng, Q.X. (1998). White spot syndrome virus infection of cultured shrimp in China. Journal of aquatic animal health, 10(4):405-410.
Downloads
Submitted
Published
Issue
Section
License
All the copy right belongs to the sponsoring Organization, Tamil Nadu Veterinary and Animal Sciences University, Chennai - 51.