CHARACTERISATION OF BOVINE LYMPHOCYTE ANTIGEN BOLA-DRB3.2 ALLELES IN INDIAN ONGOLE (Bos indicus) CATTLE BY PCR-RFLP AND PCR-SBT
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Authors
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R. Saravanan
Assistant Professor, Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal
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N. Murali
Professor and Head, Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal
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D.N. Das
Principal Scientist & I/C Genetics Laboratory, Dairy Production Section Southern Regional Station, ICAR - National Dairy Research Institute, Adugodi, Bangalore- 560 030
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A.K. Thiruvenkadan
Professor, Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal
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S.Velusamy
Senior Research Fellow, Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal
Abstract
Bovine Lymphocyte Antigen DRB3 (BoLA-DRB3), a gene of the major histocompatibility complex (MHC) has received attention because this is considered to be a potential genetic marker associated with disease resistance traits in cattle. Hence, a study was taken up to
characterize BoLA-DRB3.2 alleles in the 60 Ongole animals. The most common alleles observed in Ongole cattle was *15 and *6 with frequency of 0.225 and 0.200, respectively. Of the 22 identified alleles detedted, these 7 allleles (BoLA-DRB3.2*15,*6,*12,*13,*23, *31 and *47) represented 77.5 percent of allelic frequencies. Result indicated that the BoLADRB3 exon 2 was highly polymorphic in Ongole cattle. Direct sequencing BoLA-DRB3.2 allele reveled that at nucleotide at position 91 and 133 of the sequence had both A and G nucleotides in contrast to T nucleotide of Bos taurus breed and the variations leads to different band pattern in gels.
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References
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