DETECTION OF MYCOBACTERIUM SPP. IN DUNG PELLETS OF SPOTTED DEER (AXIS AXIS) BY PCR

G. Sathish; A. ShanmugaSundaram; M. Parthiban; C. Sreekumar

doi:10.56093/ijvasr.v55i4.181112

Authors

G. Sathish Ph.D. Scholar, Department of Animal Biotechnology, Madras Veterinary College, Chennai - 600 007
A. ShanmugaSundaram Assistant Professor, Department of Wildlife Science, Faculty of Basic Sciences, Madras Veterinary College, Chennai - 600 007
M. Parthiban Ph.D. Scholar, Department of Animal Biotechnology, Madras Veterinary College, Chennai - 600 007
C. Sreekumar Professor and Head, Department of Wild life Sciences, Madras Veterinary College, Chennai - 600 007

https://doi.org/10.56093/ijvasr.v55i4.181112

Keywords:

Mycobacterium tuberculosis complex, faecal pellets, spotted deer, duplex PCR

Abstract

The study was conducted to investigate the presence of Mycobacterium spp. in dung of spotted deer (Axis axis). Non-invasive sample collection methods were followed to avoid stress. Genomic DNA was extracted from stool samples of spotted deer from Indian Institute of Technology-Madras (IIT-M) and Arignar Anna Zoological Park (AAZP), Vandalur. Samples were collected from five and four different sites of IIT-M and AAZP, Vandalur respectively. The pooled samples from each site were utilized for genomic DNA extraction. Duplex PCR with specific primers, were used to differentiate MTB (M. tuberculosis, M. bovis) and NTB (M. avium). The five samples from IIT-M amplified fragments specific to M. tuberculosis and M. bovis. All the samples collected from AAZP, Vandalur were negative. As the PCR results are presumptive, the PCR positivity of IIT-M samples should be further investigated and confirmed individually with other molecular techniques like sequencing to understand the exact species/genotype circulating in urban wildlife.

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